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Alternative name
DNA topoisomerase 2-alpha ELISA KIT; DNA topoisomerase II, alpha isozymeTOP2A ELISA KIT; TOP2 ELISA KIT
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Catalog
E012339
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species
Human
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GeneDSDNAAB
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman DSDNAAB ELISA Kit allows for the in vitro quantitative determination of DSDNAAB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The kit uses a double?antigen sandwich enzyme?linked immunosorbent assay (ELISA) to analyze the existence or not of Human DSDNAAB in samples. Add sample to wells pre?coated with one Human Double Stranded DNA antigen, at same time add HRP-conjugated Human Double Stranded DNA antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human DSDNAAB in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.
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