Human DAT ELISA Kit

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  • Alternative name

    Sodium-dependent dopamine transporter ELISA KIT; Solute carrier family 6 member 3SLC6A3 ELISA KIT; DAT1 ELISA KIT; DA transporter ELISA KIT; DAT ELISA KIT

  • Catalog
    E012323
  • species
    Human
  • GeneDAT
  • Standard CurveHuman DAT ELISA Kit
  • Other Species Mouse DAT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DAT . No significant cross-reactivity or interference between DAT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.094ng/ml
  • Intended UseHuman DAT ELISA Kit allows for the in vitro quantitative determination of DAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DAT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DAT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DAT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DAT can be calculated.


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