Human DRD2 ELISA Kit

Contact Us
  • Alternative name

    Human dopamine D2 receptor ELISA Kit;Human D2DR ELISA Kit;Human D2R ELISA Kit;Human dopamine receptor D2 ELISA Kit;Human D(2) dopamine receptor ELISA Kit;Human dopamine receptor D2 isoform ELISA Kit;Human seven transmembrane helix receptor ELISA Kit;

  • Catalog
    E012313
  • species
    Human
  • GeneDRD2
  • Standard CurveHuman DRD2 ELISA Kit
  • Other Species Human D2R/DRD2 ELISA KitMouse Drd2 ELISA KitBovine DRD2 ELISA KitCanine DRD2 ELISA KitTurkey DRD2 ELISA KitRat Drd2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DRD2 ELISA Kit allows for the in vitro quantitative determination of DRD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This DRD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRD2. This ELISA kit for researchuse only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY DRD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD2 antibody and an DRD2-HRP conjugate. The assay sample and buffer are incubated together with DRD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD2 concentration since DRD2 from samples and DRD2-HRP conjugate compete for the anti-DRD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD2 from the sample, fewer sites are left to bind DRD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD2 concentration in each sample is interpolated from this standard curve.
  • Human D(2) dopamine receptor Protein information
  • Uniprot ID DRD2_HUMAN
  • Uniprot AC P14416; Q9NZR3; Q9UPA9;
  • UniGene Hs.73893;
  • GeneID 1813
  • KEGG hsa:1813;
  • Human D(2) dopamine receptor Protein SEQUENCE
  • SEQUENCE 443 AA; 50619 MW; 9BF8EA36C988A2E2 CRC64;

    MDPLNLSWYD DDLERQNWSR PFNGSDGKAD RPHYNYYATL LTLLIAVIVF

    GNVLVCMAVS REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW

    KFSRIHCDIF VTLDVMMCTA SILNLCAISI DRYTAVAMPM LYNTRYSSKR

    RVTVMISIVW VLSFTISCPL LFGLNNADQN ECIIANPAFV VYSSIVSFYV

    PFIVTLLVYI KIYIVLRRRR KRVNTKRSSR AFRAHLRAPL KGNCTHPEDM

    KLCTVIMKSN GSFPVNRRRV EAARRAQELE MEMLSSTSPP ERTRYSPIPP

    SHHQLTLPDP SHHGLHSTPD SPAKPEKNGH AKDHPKIAKI FEIQTMPNGK

    TRTSLKTMSR RKLSQQKEKK ATQMLAIVLG VFIICWLPFF ITHILNIHCD

    CNIPPVLYSA FTWLGYVNSA VNPIIYTTFN IEFRKAFLKI LHC

  • UCSC uc001pnz.4; human. [P14416-1];



Inquiry About Human DRD2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!