Human DBH ELISA Kit

Alternative name
Human Dopamine beta-monooxygenase ELISA Kit;Human DBM ELISA Kit;Human dopamine beta-hydroxylase ELISA Kit;Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit;
Catalog
E012296
species
Human
GeneDBH
Standard Curve
Other Species Mouse Dbh ELISA Kit Bovine DBH ELISA Kit Canine DBH ELISA Kit Horse DBH ELISA Kit Rat Dbh ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dopamine Beta Hydroxylase (DbH). No significant cross-reactivity or interference between Dopamine Beta Hydroxylase (DbH) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 1.18ng/mL.
Intended UseHuman DBH ELISA Kit allows for the in vitro quantitative determination of DBH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Beta Hydroxylase (DbH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Beta Hydroxylase (DbH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Beta Hydroxylase (DbH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Beta Hydroxylase (DbH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Soluble dopamine beta-hydroxylase Protein information
Uniprot ID DOPO_HUMAN
Uniprot AC P09172; Q5T381; Q96AG2;
UniGene Hs.591890;
GeneID 1621
KEGG hsa:1621;
Human Soluble dopamine beta-hydroxylase Protein SEQUENCE
SEQUENCE 617 AA; 69065 MW; 4D5586F0E358885D CRC64;
MPALSRWASL PGPSMREAAF MYSTAVAIFL VILVAALQGS APRESPLPYH
IPLDPEGSLE LSWNVSYTQE AIHFQLLVRR LKAGVLFGMS DRGELENADL
VVLWTDGDTA YFADAWSDQK GQIHLDPQQD YQLLQVQRTP EGLTLLFKRP
FGTCDPKDYL IEDGTVHLVY GILEEPFRSL EAINGSGLQM GLQRVQLLKP
NIPEPELPSD ACTMEVQAPN IQIPSQETTY WCYIKELPKG FSRHHIIKYE
PIVTKGNEAL VHHMEVFQCA PEMDSVPHFS GPCDSKMKPD RLNYCRHVLA
AWALGAKAFY YPEEAGLAFG GPGSSRYLRL EVHYHNPLVI EGRNDSSGIR
LYYTAKLRRF NAGIMELGLV YTPVMAIPPR ETAFILTGYC TDKCTQLALP
PSGIHIFASQ LHTHLTGRKV VTVLVRDGRE WEIVNQDNHY SPHFQEIRML
KKVVSVHPGD VLITSCTYNT EDRELATVGG FGILEEMCVN YVHYYPQTQL
ELCKSAVDAG FLQKYFHLIN RFNNEDVCTC PQASVSQQFT SVPWNSFNRD
VLKALYSFAP ISMHCNKSSA VRFQGEWNLQ PLPKVISTLE EPTPQCPTSQ
GRSPAGPTVV SIGGGKG
UCSC uc004cel.4; human.;

Related products with Human DBH ELISA Kit

Category

Human DBH ELISA Kit

Human Dopamine beta-monooxygenase ELISA Kit;Human DBM ELISA Kit;Human dopamine beta-hydroxylase ELISA Kit;Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit;