Human DBH ELISA Kit

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  • Alternative name

    Human Dopamine beta-monooxygenase ELISA Kit;Human DBM ELISA Kit;Human dopamine beta-hydroxylase ELISA Kit;Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit;

  • Catalog
    E012296
  • species
    Human
  • GeneDBH
  • Standard CurveHuman DBH ELISA Kit
  • Other Species Mouse Dbh ELISA KitBovine DBH ELISA KitCanine DBH ELISA KitHorse DBH ELISA KitRat Dbh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dopamine Beta Hydroxylase (DbH). No significant cross-reactivity or interference between Dopamine Beta Hydroxylase (DbH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.18ng/mL.
  • Intended UseHuman DBH ELISA Kit allows for the in vitro quantitative determination of DBH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Beta Hydroxylase (DbH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Beta Hydroxylase (DbH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Beta Hydroxylase (DbH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Beta Hydroxylase (DbH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Soluble dopamine beta-hydroxylase Protein information
  • Uniprot ID DOPO_HUMAN
  • Uniprot AC P09172; Q5T381; Q96AG2;
  • UniGene Hs.591890;
  • GeneID 1621
  • KEGG hsa:1621;
  • Human Soluble dopamine beta-hydroxylase Protein SEQUENCE
  • SEQUENCE 617 AA; 69065 MW; 4D5586F0E358885D CRC64;

    MPALSRWASL PGPSMREAAF MYSTAVAIFL VILVAALQGS APRESPLPYH

    IPLDPEGSLE LSWNVSYTQE AIHFQLLVRR LKAGVLFGMS DRGELENADL

    VVLWTDGDTA YFADAWSDQK GQIHLDPQQD YQLLQVQRTP EGLTLLFKRP

    FGTCDPKDYL IEDGTVHLVY GILEEPFRSL EAINGSGLQM GLQRVQLLKP

    NIPEPELPSD ACTMEVQAPN IQIPSQETTY WCYIKELPKG FSRHHIIKYE

    PIVTKGNEAL VHHMEVFQCA PEMDSVPHFS GPCDSKMKPD RLNYCRHVLA

    AWALGAKAFY YPEEAGLAFG GPGSSRYLRL EVHYHNPLVI EGRNDSSGIR

    LYYTAKLRRF NAGIMELGLV YTPVMAIPPR ETAFILTGYC TDKCTQLALP

    PSGIHIFASQ LHTHLTGRKV VTVLVRDGRE WEIVNQDNHY SPHFQEIRML

    KKVVSVHPGD VLITSCTYNT EDRELATVGG FGILEEMCVN YVHYYPQTQL

    ELCKSAVDAG FLQKYFHLIN RFNNEDVCTC PQASVSQQFT SVPWNSFNRD

    VLKALYSFAP ISMHCNKSSA VRFQGEWNLQ PLPKVISTLE EPTPQCPTSQ

    GRSPAGPTVV SIGGGKG

  • UCSC uc004cel.4; human.;


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