Human DCT ELISA Kit

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  • Alternative name

    Human DCT ELISA Kit;Human DT ELISA Kit;Human L-dopachrome Delta-isomerase ELISA Kit;Human Tyrosinase-related protein 2 ELISA Kit;Human TRP-2 ELISA Kit;Human TRP2 ELISA Kit;Human TYRP2 ELISA Kit;Human dopachrome tautomerase ELISA Kit;Human L-dopachrome tautomerase ELISA Kit;Human L-dopachrome isomerase ELISA Kit;Human dopachrome delta-isomerase ELISA Kit;Human dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) ELISA Kit;Human tyrosinase related protein-2 ELISA Kit;Human tyrosine-related protein 2 ELISA Kit;

  • Catalog
    E012284
  • species
    Human
  • GeneDCT
  • Standard CurveHuman DCT ELISA Kit
  • Other Species Mouse Dct ELISA KitMouse 11-DCT ELISA KitBovine DCT ELISA KitChicken DCT ELISA KitPorcine DCT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dopachrome Tautomerase (DCT). No significant cross-reactivity or interference between Dopachrome Tautomerase (DCT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.128ng/mL.
  • Intended UseHuman DCT ELISA Kit allows for the in vitro quantitative determination of DCT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopachrome Tautomerase (DCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopachrome Tautomerase (DCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopachrome Tautomerase (DCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopachrome Tautomerase (DCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human L-dopachrome tautomerase Protein information
  • Uniprot ID TYRP2_HUMAN
  • Uniprot AC P40126; Q09GT4;
  • UniGene Hs.301865;
  • GeneID 1638
  • KEGG hsa:1638;
  • Human L-dopachrome tautomerase Protein SEQUENCE
  • SEQUENCE 519 AA; 59145 MW; AFDDF21768002A89 CRC64;

    MSPLWWGFLL SCLGCKILPG AQGQFPRVCM TVDSLVNKEC CPRLGAESAN

    VCGSQQGRGQ CTEVRADTRP WSGPYILRNQ DDRELWPRKF FHRTCKCTGN

    FAGYNCGDCK FGWTGPNCER KKPPVIRQNI HSLSPQEREQ FLGALDLAKK

    RVHPDYVITT QHWLGLLGPN GTQPQFANCS VYDFFVWLHY YSVRDTLLGP

    GRPYRAIDFS HQGPAFVTWH RYHLLCLERD LQRLIGNESF ALPYWNFATG

    RNECDVCTDQ LFGAARPDDP TLISRNSRFS SWETVCDSLD DYNHLVTLCN

    GTYEGLLRRN QMGRNSMKLP TLKDIRDCLS LQKFDNPPFF QNSTFSFRNA

    LEGFDKADGT LDSQVMSLHN LVHSFLNGTN ALPHSAANDP IFVVLHSFTD

    AIFDEWMKRF NPPADAWPQE LAPIGHNRMY NMVPFFPPVT NEELFLTSDQ

    LGYSYAIDLP VSVEETPGWP TTLLVVMGTL VALVGLFVLL AFLQYRRLRK

    GYTPLMETHL SSKRYTEEA

  • UCSC uc001vlv.6; human. [P40126-1];


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