Human DT ELISA Kit

Contact Us
  • Alternative name

    L-dopachrome tautomerase ELISA KIT; L-dopachrome Delta-isomerase ELISA KIT; Tyrosinase-related protein 2 ELISA KIT; TRP-2 ELISA KIT; TRP2DCT ELISA KIT; TYRP2 ELISA KIT; DCT ELISA KIT; DT ELISA KIT; TRP-2 ELISA KIT; TRP2 ELISA KIT

  • Catalog
    E012282
  • species
    Human
  • GeneDT
  • Standard CurveHuman DT ELISA Kit
  • Other Species Mouse DT ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DT ELISA Kit allows for the in vitro quantitative determination of DT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: DT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DT antibody and an DT-HRP conjugate. The assay sample and buffer are incubated together with DT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DT concentration since DT from samples and DT-HRP conjugate compete for the anti-DT antibody binding site. Since the number of sites is limited, as more sites are occupied by DT from the sample, fewer sites are left to bind DT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DT concentration in each sample is interpolated from this standard curve.


Related products with Human DT ELISA Kit

Inquiry About Human DT ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!