Human DDOST ELISA Kit

Alternative name
Human DDOST 48 kDa subunit ELISA Kit;Human oligosaccharyl transferase 48 kDa subunit ELISA Kit;Human KIAA0115 ELISA Kit;Human OST48 ELISA Kit;Human AGER1 ELISA Kit;Human CDG1R ELISA Kit;Human OKSWcl45 ELISA Kit;Human OST ELISA Kit;Human WBP1 ELISA Kit;Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit ELISA Kit;Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit;Human advanced glycation end-product receptor 1 ELISA Kit;Human advanced glycation endproduct receptor 1 ELISA Kit;Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) ELISA Kit;Human dolichyl-diphosphooligosaccharide-protein glycotransferase ELISA Kit;Human oligosaccharyltransferase 48 kDa subunit ELISA Kit;Human oligosaccharyltransferase subunit 48 ELISA Kit;
Catalog
E012274
species
Human
GeneDDOST
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human DDOST. No significant cross-reactivity or interference between Human DDOST and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.078 ng/ml
Intended UseHuman DDOST ELISA Kit allows for the in vitro quantitative determination of DDOST , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDOST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DDOST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain DDOST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DDOST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein information
Uniprot ID OST48_HUMAN
Uniprot AC P39656; B2RDQ4; B4DJE3; B4DLI2; O43244; Q5VWA5; Q8NI93; Q9BUI2;
UniGene Hs.523145;
GeneID 1650
KEGG hsa:1650;
Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein SEQUENCE
SEQUENCE 456 AA; 50801 MW; 2707ABAC8FF158CB CRC64;
MGYFRCARAG SFGRRRKMEP STAARAWALF WLLLPLLGAV CASGPRTLVL
LDNLNVRETH SLFFRSLKDR GFELTFKTAD DPSLSLIKYG EFLYDNLIIF
SPSVEDFGGN INVETISAFI DGGGSVLVAA SSDIGDPLRE LGSECGIEFD
EEKTAVIDHH NYDISDLGQH TLIVADTENL LKAPTIVGKS SLNPILFRGV
GMVADPDNPL VLDILTGSST SYSFFPDKPI TQYPHAVGKN TLLIAGLQAR
NNARVIFSGS LDFFSDSFFN SAVQKAAPGS QRYSQTGNYE LAVALSRWVF
KEEGVLRVGP VSHHRVGETA PPNAYTVTDL VEYSIVIQQL SNGKWVPFDG
DDIQLEFVRI DPFVRTFLKK KGGKYSVQFK LPDVYGVFQF KVDYNRLGYT
HLYSSTQVSV RPLQHTQYER FIPSAYPYYA SAFSMMLGLF IFSIVFLHMK
EKEKSD
UCSC uc001bdo.1; human. [P39656-1];

Category

Human DDOST ELISA Kit