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Alternative name
DNA (cytosine-5)-methyltransferase 3B ELISA KIT; DNA methyltransferase HsaIIIB ELISA KIT; DNA MTase HsaIIIB ELISA KIT; M.HsaIIIBDNMT3B ELISA KIT; Dnmt3b ELISA KIT; DNA MTase HsaIIIB ELISA KIT; M.HsaIIIB ELISA KIT
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Catalog
E012147
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species
Human
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GeneDNMT3B
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SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 3B (DNMT3B).
No significant cross-reactivity or interference between DNA Methyltransferase 3B (DNMT3B) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.112ng/mL.
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Intended UseHuman DNMT3B ELISA Kit allows for the in vitro quantitative determination of DNMT3B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 3B (DNMT3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 3B (DNMT3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 3B (DNMT3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 3B (DNMT3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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