Human DNMT1 ELISA Kit

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  • Alternative name

    Human Dnmt1 ELISA Kit;Human CXXC-type zinc finger protein 9 ELISA Kit;Human DNA methyltransferase HsaI ELISA Kit;Human DNA MTase HsaI ELISA Kit;Human m.HsaI ELISA Kit;Human MCMT ELISA Kit;Human AIM ELISA Kit;Human CXXC9 ELISA Kit;Human DNMT ELISA Kit;Human ADCADN ELISA Kit;Human HSN1E ELISA Kit;Human DNA methyltransferase 1 ELISA Kit;Human DNA (cytosine-5)-methyltransferase 1 ELISA Kit;Human DNA (cytosine-5-)-methyltransferase 1 ELISA Kit;

  • Catalog
    E012141
  • species
    Human
  • GeneDNMT1
  • Standard CurveHuman DNMT1 ELISA Kit
  • Other Species Mouse Dnmt1 ELISA KitBovine DNMT1 ELISA KitChicken DNMT1 ELISA KitRat Dnmt1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 1 (DNMT1). No significant cross-reactivity or interference between DNA Methyltransferase 1 (DNMT1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman DNMT1 ELISA Kit allows for the in vitro quantitative determination of DNMT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 1 (DNMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 1 (DNMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 1 (DNMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 1 (DNMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA (cytosine-5)-methyltransferase 1 Protein information
  • Uniprot ID DNMT1_HUMAN
  • Uniprot AC P26358; A0AV63; B7ZLW6; Q9UHG5; Q9ULA2; Q9UMZ6;
  • UniGene Hs.202672;
  • GeneID 1786
  • KEGG hsa:1786;
  • Human DNA (cytosine-5)-methyltransferase 1 Protein SEQUENCE
  • SEQUENCE 1616 AA; 183165 MW; 1E833192D22AFA5B CRC64;

    MPARTAPARV PTLAVPAISL PDDVRRRLKD LERDSLTEKE CVKEKLNLLH

    EFLQTEIKNQ LCDLETKLRK EELSEEGYLA KVKSLLNKDL SLENGAHAYN

    REVNGRLENG NQARSEARRV GMADANSPPK PLSKPRTPRR SKSDGEAKPE

    PSPSPRITRK STRQTTITSH FAKGPAKRKP QEESERAKSD ESIKEEDKDQ

    DEKRRRVTSR ERVARPLPAE EPERAKSGTR TEKEEERDEK EEKRLRSQTK

    EPTPKQKLKE EPDREARAGV QADEDEDGDE KDEKKHRSQP KDLAAKRRPE

    EKEPEKVNPQ ISDEKDEDEK EEKRRKTTPK EPTEKKMARA KTVMNSKTHP

    PKCIQCGQYL DDPDLKYGQH PPDAVDEPQM LTNEKLSIFD ANESGFESYE

    ALPQHKLTCF SVYCKHGHLC PIDTGLIEKN IELFFSGSAK PIYDDDPSLE

    GGVNGKNLGP INEWWITGFD GGEKALIGFS TSFAEYILMD PSPEYAPIFG

    LMQEKIYISK IVVEFLQSNS DSTYEDLINK IETTVPPSGL NLNRFTEDSL

    LRHAQFVVEQ VESYDEAGDS DEQPIFLTPC MRDLIKLAGV TLGQRRAQAR

    RQTIRHSTRE KDRGPTKATT TKLVYQIFDT FFAEQIEKDD REDKENAFKR

    RRCGVCEVCQ QPECGKCKAC KDMVKFGGSG RSKQACQERR CPNMAMKEAD

    DDEEVDDNIP EMPSPKKMHQ GKKKKQNKNR ISWVGEAVKT DGKKSYYKKV

    CIDAETLEVG DCVSVIPDDS SKPLYLARVT ALWEDSSNGQ MFHAHWFCAG

    TDTVLGATSD PLELFLVDEC EDMQLSYIHS KVKVIYKAPS ENWAMEGGMD

    PESLLEGDDG KTYFYQLWYD QDYARFESPP KTQPTEDNKF KFCVSCARLA

    EMRQKEIPRV LEQLEDLDSR VLYYSATKNG ILYRVGDGVY LPPEAFTFNI

    KLSSPVKRPR KEPVDEDLYP EHYRKYSDYI KGSNLDAPEP YRIGRIKEIF

    CPKKSNGRPN ETDIKIRVNK FYRPENTHKS TPASYHADIN LLYWSDEEAV

    VDFKAVQGRC TVEYGEDLPE CVQVYSMGGP NRFYFLEAYN AKSKSFEDPP

    NHARSPGNKG KGKGKGKGKP KSQACEPSEP EIEIKLPKLR TLDVFSGCGG

    LSEGFHQAGI SDTLWAIEMW DPAAQAFRLN NPGSTVFTED CNILLKLVMA

    GETTNSRGQR LPQKGDVEML CGGPPCQGFS GMNRFNSRTY SKFKNSLVVS

    FLSYCDYYRP RFFLLENVRN FVSFKRSMVL KLTLRCLVRM GYQCTFGVLQ

    AGQYGVAQTR RRAIILAAAP GEKLPLFPEP LHVFAPRACQ LSVVVDDKKF

    VSNITRLSSG PFRTITVRDT MSDLPEVRNG ASALEISYNG EPQSWFQRQL

    RGAQYQPILR DHICKDMSAL VAARMRHIPL APGSDWRDLP NIEVRLSDGT

    MARKLRYTHH DRKNGRSSSG ALRGVCSCVE AGKACDPAAR QFNTLIPWCL

    PHTGNRHNHW AGLYGRLEWD GFFSTTVTNP EPMGKQGRVL HPEQHRVVSV

    RECARSQGFP DTYRLFGNIL DKHRQVGNAV PPPLAKAIGL EIKLCMLAKA

    RESASAKIKE EEAAKD

  • UCSC uc002mng.4; human. [P26358-1];


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