Human LIG3 ELISA Kit

Alternative name
Human DNA ligase III ELISA Kit;Human polydeoxyribonucleotide synthase [ATP] 3 ELISA Kit;Human LIG2 ELISA Kit;Human DNA ligase 3 ELISA Kit;Human ligase II, DNA, ATP-dependent ELISA Kit;Human ligase III, DNA, ATP-dependent ELISA Kit;
Catalog
E012137
species
Human
GeneLIG3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman LIG3 ELISA Kit allows for the in vitro quantitative determination of LIG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LIG3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LIG3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LIG3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LIG3 can be calculated.
Human DNA ligase 3 Protein information
Uniprot ID DNLI3_HUMAN
Uniprot AC P49916; E5KLB5; E5KLB6; Q16714; Q6NVK3;
UniGene Hs.100299; Hs.556108;
GeneID 3980
KEGG hsa:3980;
Human DNA ligase 3 Protein SEQUENCE
SEQUENCE 1009 AA; 112907 MW; 0E4057E33C3F19A6 CRC64;
MSLAFKIFFP QTLRALSRKE LCLFRKHHWR DVRQFSQWSE TDLLHGHPLF
LRRKPVLSFQ GSHLRSRATY LVFLPGLHVG LCSGPCEMAE QRFCVDYAKR
GTAGCKKCKE KIVKGVCRIG KVVPNPFSES GGDMKEWYHI KCMFEKLERA
RATTKKIEDL TELEGWEELE DNEKEQITQH IADLSSKAAG TPKKKAVVQA
KLTTTGQVTS PVKGASFVTS TNPRKFSGFS AKPNNSGEAP SSPTPKRSLS
SSKCDPRHKD CLLREFRKLC AMVADNPSYN TKTQIIQDFL RKGSAGDGFH
GDVYLTVKLL LPGVIKTVYN LNDKQIVKLF SRIFNCNPDD MARDLEQGDV
SETIRVFFEQ SKSFPPAAKS LLTIQEVDEF LLRLSKLTKE DEQQQALQDI
ASRCTANDLK CIIRLIKHDL KMNSGAKHVL DALDPNAYEA FKASRNLQDV
VERVLHNAQE VEKEPGQRRA LSVQASLMTP VQPMLAEACK SVEYAMKKCP
NGMFSEIKYD GERVQVHKNG DHFSYFSRSL KPVLPHKVAH FKDYIPQAFP
GGHSMILDSE VLLIDNKTGK PLPFGTLGVH KKAAFQDANV CLFVFDCIYF
NDVSLMDRPL CERRKFLHDN MVEIPNRIMF SEMKRVTKAL DLADMITRVI
QEGLEGLVLK DVKGTYEPGK RHWLKVKKDY LNEGAMADTA DLVVLGAFYG
QGSKGGMMSI FLMGCYDPGS QKWCTVTKCA GGHDDATLAR LQNELDMVKI
SKDPSKIPSW LKVNKIYYPD FIVPDPKKAA VWEITGAEFS KSEAHTADGI
SIRFPRCTRI RDDKDWKSAT NLPQLKELYQ LSKEKADFTV VAGDEGSSTT
GGSSEENKGP SGSAVSRKAP SKPSASTKKA EGKLSNSNSK DGNMQTAKPS
AMKVGEKLAT KSSPVKVGEK RKAADETLCQ TKVLLDIFTG VRLYLPPSTP
DFSRLRRYFV AFDGDLVQEF DMTSATHVLG SRDKNPAAQQ VSPEWIWACI
RKRRLVAPC
UCSC uc002hij.4; human. [P49916-1];

Category

Human LIG3 ELISA Kit

Human DNA ligase III ELISA Kit;Human polydeoxyribonucleotide synthase [ATP] 3 ELISA Kit;Human LIG2 ELISA Kit;Human DNA ligase 3 ELISA Kit;Human ligase II, DNA, ATP-dependent ELISA Kit;Human ligase III, DNA, ATP-dependent ELISA Kit;