Human ID2 ELISA Kit

Alternative name
Human class B basic helix-loop-helix protein 26 ELISA Kit;Human bHLHb26 ELISA Kit;Human Inhibitor of DNA binding 2 ELISA Kit;Human inhibitor of differentiation 2 ELISA Kit;Human GIG8 ELISA Kit;Human ID2A ELISA Kit;Human ID2H ELISA Kit;Human inhibitor of DNA binding 2, HLH protein ELISA Kit;Human DNA-binding protein inhibitor ID-2 ELISA Kit;Human DNA-binding protein inhibitor ID2 ELISA Kit;Human cell growth-inhibiting gene 8 ELISA Kit;Human helix-loop-helix protein ID2 ELISA Kit;Human inhibitor of DNA binding 2, dominant negative helix-loop-helix protein ELISA Kit;
Catalog
E012119
species
Human
GeneID2
SpecificityThis assay has high sensitivity and excellent specificity for detection of ID2. No significant cross-reactivity or interference between ID2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ID2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ID2 ELISA Kit allows for the in vitro quantitative determination of ID2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: ID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ID2 antibody and an ID2-HRP conjugate. The assay sample and buffer are incubated together with ID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ID2 concentration since ID2 from samples and ID2-HRP conjugate compete for the anti-ID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ID2 from the sample, fewer sites are left to bind ID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ID2 concentration in each sample is interpolated from this standard curve.
Human DNA-binding protein inhibitor ID-2 Protein information
Uniprot ID ID2_HUMAN
Uniprot AC Q02363;
UniGene Hs.180919; Hs.726053;
GeneID 3398
KEGG hsa:3398;
Human DNA-binding protein inhibitor ID-2 Protein SEQUENCE
SEQUENCE 134 AA; 14917 MW; A0D98B96396EB11E CRC64;
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV
PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR
TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG

Human ID2 ELISA Kit

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