Human class B basic helix-loop-helix protein 26 ELISA Kit;Human bHLHb26 ELISA Kit;Human Inhibitor of DNA binding 2 ELISA Kit;Human inhibitor of differentiation 2 ELISA Kit;Human GIG8 ELISA Kit;Human ID2A ELISA Kit;Human ID2H ELISA Kit;Human inhibitor of DNA binding 2, HLH protein ELISA Kit;Human DNA-binding protein inhibitor ID-2 ELISA Kit;Human DNA-binding protein inhibitor ID2 ELISA Kit;Human cell growth-inhibiting gene 8 ELISA Kit;Human helix-loop-helix protein ID2 ELISA Kit;Human inhibitor of DNA binding 2, dominant negative helix-loop-helix protein ELISA Kit;
SpecificityThis assay has high sensitivity and excellent specificity for detection of ID2. No significant cross-reactivity or interference between ID2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ID2 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman ID2 ELISA Kit allows for the in vitro quantitative determination of ID2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description specificalPrinciple of the assay: ID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ID2 antibody and an ID2-HRP conjugate. The assay sample and buffer are incubated together with ID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ID2 concentration since ID2 from samples and ID2-HRP conjugate compete for the anti-ID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ID2 from the sample, fewer sites are left to bind ID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ID2 concentration in each sample is interpolated from this standard curve.
Human DNA-binding protein inhibitor ID-2 Protein information
Human DNA-binding protein inhibitor ID-2 Protein SEQUENCE