Human DLX5 ELISA Kit

Alternative name
Human SHFM1D ELISA Kit;Human distal-less homeobox 5 ELISA Kit;Human homeobox protein DLX-5 ELISA Kit;Human distal-less homeo box 5 ELISA Kit;Human split hand/foot malformation type 1 with sensorineural hearing loss ELISA Kit;
Catalog
E012078
species
Human
GeneDLX5
Standard Curve
Other Species Mouse Dlx5 ELISA Kit Chicken DLX5 ELISA Kit Rat Dlx5 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman DLX5 ELISA Kit allows for the in vitro quantitative determination of DLX5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: DLX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DLX5 antibody and an DLX5-HRP conjugate. The assay sample and buffer are incubated together with DLX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DLX5 concentration since DLX5 from samples and DLX5-HRP conjugate compete for the anti-DLX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by DLX5 from the sample, fewer sites are left to bind DLX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DLX5 concentration in each sample is interpolated from this standard curve.
Human Homeobox protein DLX-5 Protein information
Uniprot ID DLX5_HUMAN
Uniprot AC P56178; B7Z4P3; Q9UPL1;
UniGene Hs.99348;
GeneID 1749
KEGG hsa:1749;
Human Homeobox protein DLX-5 Protein SEQUENCE
SEQUENCE 289 AA; 31540 MW; 5BC9970B12E30554 CRC64;
MTGVFDRRVP SIRSGDFQAP FQTSAAMHHP SQESPTLPES SATDSDYYSP
TGGAPHGYCS PTSASYGKAL NPYQYQYHGV NGSAGSYPAK AYADYSYASS
YHQYGGAYNR VPSATNQPEK EVTEPEVRMV NGKPKKVRKP RTIYSSFQLA
ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKIMKNGE
MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH PPTSNQSPAS
SYLENSASWY TSAASSINSH LPPPGSLQHP LALASGTLY
UCSC uc011kim.2; human. [P56178-1];

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anti-DLX5 antibody

Category

Human DLX5 ELISA Kit

Human SHFM1D ELISA Kit;Human distal-less homeobox 5 ELISA Kit;Human homeobox protein DLX-5 ELISA Kit;Human distal-less homeo box 5 ELISA Kit;Human split hand/foot malformation type 1 with sensorineural hearing loss ELISA Kit;