Human DHT ELISA Kit

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  • Alternative name

    D-hydantoinase/dihydropyrimidinase Dht ELISA KIT; D-hydantoinase/dihydropyrimidinase Dhtdht ELISA KIT; RLO149_c023310 ELISA KIT

  • Catalog
    E011961
  • species
    Human
  • GeneDHT
  • Standard CurveHuman DHT ELISA Kit
  • Other Species Human DHT ELISA KitMouse DHT ELISA KitBovine DHT ELISA KitCanine DHT ELISA KitChicken DHT ELISA KitGeneral DHT ELISA KitPorcine DHT ELISA KitRabbit DHT ELISA KitRat DHT ELISA KitCamel DHT ELISA KitGoat DHT ELISA KitGuinea Pig DHT ELISA KitHamster DHT ELISA KitHorse DHT ELISA KitMonkey DHT ELISA KitSheep DHT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DHT. No significant cross-reactivity or interference between DHT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman DHT ELISA Kit allows for the in vitro quantitative determination of DHT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This DHT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DHT. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: DHT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DHT antibody and an DHT-HRP conjugate. The assay sample and buffer are incubated together with DHT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHT concentration since DHT from samples and DHT-HRP conjugate compete for the anti-DHT antibody binding site. Since the number of sites is limited, as more sites are occupied by DHT from the sample, fewer sites are left to bind DHT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHT concentration in each sample is interpolated from this standard curve.


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