Human DPYS ELISA Kit

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  • Alternative name

    Human DHP ELISA Kit;Human DHPase ELISA Kit;Human dihydropyrimidine amidohydrolase ELISA Kit;Human hydantoinase ELISA Kit;Human dihydropyrimidinase ELISA Kit;

  • Catalog
    E011941
  • species
    Human
  • GeneDPYS
  • Standard CurveHuman DPYS ELISA Kit
  • Other Species Mouse Dpys ELISA KitRat Dpys ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dihydropyrimidinase (DPYS). No significant cross-reactivity or interference between Dihydropyrimidinase (DPYS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.31ng/mL.
  • Intended UseHuman DPYS ELISA Kit allows for the in vitro quantitative determination of DPYS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dihydropyrimidinase Protein information
  • Uniprot ID DPYS_HUMAN
  • Uniprot AC Q14117;
  • UniGene Hs.443161;
  • GeneID 1807
  • KEGG hsa:1807;
  • Human Dihydropyrimidinase Protein SEQUENCE
  • SEQUENCE 519 AA; 56630 MW; 882E33D7C49D6ECC CRC64;

    MAAPSRLLIR GGRVVNDDFS EVADVLVEDG VVRALGHDLL PPGGAPAGLR

    VLDAAGKLVL PGGIDTHTHM QFPFMGSRSI DDFHQGTKAA LSGGTTMIID

    FAIPQKGGSL IEAFETWRSW ADPKVCCDYS LHVAVTWWSD QVKEEMKILV

    QDKGVNSFKM FMAYKDLYMV TDLELYEAFS RCKEIGAIAQ VHAENGDLIA

    EGAKKMLALG ITGPEGHELC RPEAVEAEAT LRAITIASAV NCPLYIVHVM

    SKSAAKVIAD ARRDGKVVYG EPIAASLGTD GTHYWNKEWH HAAHHVMGPP

    LRPDPSTPDF LMNLLANDDL TTTGTDNCTF NTCQKALGKD DFTKIPNGVN

    GVEDRMSVIW EKGVHSGKMD ENRFVAVTST NAAKIFNLYP RKGRIAVGSD

    ADIVIWDPKG TRTISAKTHH QAVNFNIFEG MVCHGVPLVT ISRGKVVYEA

    GVFSVTAGDG KFIPRKPFAE YIYKRIKQRD RTCTPTPVER APYKGEVATL

    KSRVTKEDAT AGTRKQAHP

  • UCSC uc003yly.5; human.;


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