Human QDPR ELISA Kit

Alternative name
Human HDHPR ELISA Kit;Human quinoid dihydropteridine reductase ELISA Kit;Human short chain dehydrogenase/reductase family 33C member 1 ELISA Kit;Human DHPR ELISA Kit;Human SDR33C1 ELISA Kit;Human PKU2 ELISA Kit;Human dihydropteridine reductase ELISA Kit;Human 6,7-dihydropteridine reductase ELISA Kit;Human testis secretory sperm-binding protein Li 236P ELISA Kit;
Catalog
E011939
species
Human
GeneQDPR
SpecificityThis assay has high sensitivity and excellent specificity for detection of QDPR. No significant cross-reactivity or interference between QDPR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between QDPR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman QDPR ELISA Kit allows for the in vitro quantitative determination of QDPR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: QDPR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-QDPR antibody and an QDPR-HRP conjugate. The assay sample and buffer are incubated together with QDPR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the QDPR concentration since QDPR from samples and QDPR-HRP conjugate compete for the anti-QDPR antibody binding site. Since the number of sites is limited, as more sites are occupied by QDPR from the sample, fewer sites are left to bind QDPR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The QDPR concentration in each sample is interpolated from this standard curve.
Human Dihydropteridine reductase Protein information
Uniprot ID DHPR_HUMAN
Uniprot AC P09417; A8K158; B3KW71; Q53F52; Q9H3M5;
UniGene Hs.75438;
GeneID 5860
KEGG hsa:5860;
Human Dihydropteridine reductase Protein SEQUENCE
SEQUENCE 244 AA; 25790 MW; 0852F9F0CA38AB1C CRC64;
MAAAAAAGEA RRVLVYGGRG ALGSRCVQAF RARNWWVASV DVVENEEASA
SIIVKMTDSF TEQADQVTAE VGKLLGEEKV DAILCVAGGW AGGNAKSKSL
FKNCDLMWKQ SIWTSTISSH LATKHLKEGG LLTLAGAKAA LDGTPGMIGY
GMAKGAVHQL CQSLAGKNSG MPPGAAAIAV LPVTLDTPMN RKSMPEADFS
SWTPLEFLVE TFHDWITGKN RPSSGSLIQV VTTEGRTELT PAYF
UCSC uc003gpd.4; human. [P09417-1];

Human QDPR ELISA Kit

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