Human DHODH ELISA Kit

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  • Alternative name

    Human DHOdehase ELISA Kit;Human dihydroorotate oxidase ELISA Kit;Human POADS ELISA Kit;Human URA1 ELISA Kit;Human dihydroorotate dehydrogenase (quinone) ELISA Kit;Human dihydroorotate dehydrogenase (quinone), mitochondrial ELISA Kit;Human human complement of yeast URA1 ELISA Kit;

  • Catalog
    E011936
  • species
    Human
  • GeneDHODH
  • Standard CurveHuman DHODH ELISA Kit
  • Other Species Mouse Dhodh ELISA KitBovine DHODH ELISA KitRat Dhodh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dihydroorotate Dehydrogenase (DHODH). No significant cross-reactivity or interference between Dihydroorotate Dehydrogenase (DHODH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman DHODH ELISA Kit allows for the in vitro quantitative determination of DHODH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydroorotate Dehydrogenase (DHODH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydroorotate Dehydrogenase (DHODH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydroorotate Dehydrogenase (DHODH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydroorotate Dehydrogenase (DHODH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dihydroorotate dehydrogenase (quinone), mitochondrial Protein information
  • Uniprot ID PYRD_HUMAN
  • Uniprot AC Q02127; A8K8C8; Q6P176;
  • UniGene Hs.654427;
  • GeneID 1723
  • KEGG hsa:1723;
  • Human Dihydroorotate dehydrogenase (quinone), mitochondrial Protein SEQUENCE
  • SEQUENCE 395 AA; 42867 MW; 072C3169E78C6440 CRC64;

    MAWRHLKKRA QDAVIILGGG GLLFASYLMA TGDERFYAEH LMPTLQGLLD

    PESAHRLAVR FTSLGLLPRA RFQDSDMLEV RVLGHKFRNP VGIAAGFDKH

    GEAVDGLYKM GFGFVEIGSV TPKPQEGNPR PRVFRLPEDQ AVINRYGFNS

    HGLSVVEHRL RARQQKQAKL TEDGLPLGVN LGKNKTSVDA AEDYAEGVRV

    LGPLADYLVV NVSSPNTAGL RSLQGKAELR RLLTKVLQER DGLRRVHRPA

    VLVKIAPDLT SQDKEDIASV VKELGIDGLI VTNTTVSRPA GLQGALRSET

    GGLSGKPLRD LSTQTIREMY ALTQGRVPII GVGGVSSGQD ALEKIRAGAS

    LVQLYTALTF WGPPVVGKVK RELEALLKEQ GFGGVTDAIG ADHRR

  • UCSC uc002fbp.4; human.;


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