Human DHFR ELISA Kit

Alternative name
Human DHFRP1 ELISA Kit;Human DYR ELISA Kit;Human dihydrofolate reductase ELISA Kit;
Catalog
E011922
species
Human
GeneDHFR
SpecificityThis assay has high sensitivity and excellent specificity for detection of DHFR. No significant cross-reactivity or interference between DHFR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHFR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DHFR ELISA Kit allows for the in vitro quantitative determination of DHFR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: DHFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DHFR antibody and an DHFR-HRP conjugate. The assay sample and buffer are incubated together with DHFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHFR concentration since DHFR from samples and DHFR-HRP conjugate compete for the anti-DHFR antibody binding site. Since the number of sites is limited, as more sites are occupied by DHFR from the sample, fewer sites are left to bind DHFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHFR concentration in each sample is interpolated from this standard curve.
Human Dihydrofolate reductase Protein information
Uniprot ID DYR_HUMAN
Uniprot AC P00374; B4DDD2; Q14130; Q6IRW8;
UniGene Hs.592364; Hs.648635;
GeneID 1719
KEGG hsa:1719;
Human Dihydrofolate reductase Protein SEQUENCE
SEQUENCE 187 AA; 21453 MW; EBDF3D1EC73E1566 CRC64;
MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL
VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL
TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP
EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
UCSC uc003kgy.2; human. [P00374-1];

Category

Human DHFR ELISA Kit

Human DHFRP1 ELISA Kit;Human DYR ELISA Kit;Human dihydrofolate reductase ELISA Kit;