Human DKK4 ELISA Kit

Alternative name
Human dickkopf-4 ELISA Kit;Human DKK-4 ELISA Kit;Human hDkk-4 ELISA Kit;Human dickkopf WNT signaling pathway inhibitor 4 ELISA Kit;Human dickkopf-related protein 4 ELISA Kit;
Catalog
E011894
species
Human
GeneDKK4
Standard Curve
Other Species Mouse Dkk4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 4 (DKK4). No significant cross-reactivity or interference between Dickkopf Related Protein 4 (DKK4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.067ng/mL.
Intended UseHuman DKK4 ELISA Kit allows for the in vitro quantitative determination of DKK4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 4 (DKK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 4 (DKK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 4 (DKK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 4 (DKK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dickkopf-related protein 4 short form Protein information
Uniprot ID DKK4_HUMAN
Uniprot AC Q9UBT3; Q3KNX0; Q9Y4C3;
UniGene Hs.159311;
GeneID 27121
KEGG hsa:27121;
Human Dickkopf-related protein 4 short form Protein SEQUENCE
SEQUENCE 224 AA; 24876 MW; 45F8EBC476961357 CRC64;
MVAAVLLGLS WLCSPLGALV LDFNNIRSSA DLHGARKGSQ CLSDTDCNTR
KFCLQPRDEK PFCATCRGLR RRCQRDAMCC PGTLCVNDVC TTMEDATPIL
ERQLDEQDGT HAEGTTGHPV QENQPKRKPS IKKSQGRKGQ EGESCLRTFD
CGPGLCCARH FWTKICKPVL LEGQVCSRRG HKDTAQAPEI FQRCDCGPGL
LCRSQLTSNR QHARLRVCQK IEKL
UCSC uc003xpb.4; human.;

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Human DKK4 ELISA Kit

Human dickkopf-4 ELISA Kit;Human DKK-4 ELISA Kit;Human hDkk-4 ELISA Kit;Human dickkopf WNT signaling pathway inhibitor 4 ELISA Kit;Human dickkopf-related protein 4 ELISA Kit;