Human DICER1 ELISA Kit

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  • Alternative name

    Endoribonuclease Dicer ELISA KIT; Helicase with RNase motif ELISA KIT; Helicase MOIDICER1 ELISA KIT; DICER ELISA KIT; HERNA ELISA KIT; KIAA0928 ELISA KIT; Helicase MOI ELISA KIT

  • Catalog
    E011881
  • species
    Human
  • GeneDICER1
  • Standard CurveHuman DICER1 ELISA Kit
  • Other Species Mouse Dicer1 ELISA KitBovine DICER1 ELISA KitChicken DICER1 ELISA KitHamster DICER1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dicer 1, Ribonuclease Type III (DICER1). No significant cross-reactivity or interference between Dicer 1, Ribonuclease Type III (DICER1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.119ng/mL.
  • Intended UseHuman DICER1 ELISA Kit allows for the in vitro quantitative determination of DICER1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dicer 1, Ribonuclease Type III (DICER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dicer 1, Ribonuclease Type III (DICER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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