SensitivityThe minimum detectable dose of DBI is typically less than 215.6pmol/L. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by subtractin
Intended UseHuman DBI ELISA Kit allows for the in vitro quantitative determination of DBI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DBI has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DBI and unlabeled DBI (Standards or samples) with the pre-coated antibody specific to DBI. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DBI in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DBI in the sample.
Human Acyl-CoA-binding protein Protein information