Human DGAT2 ELISA Kit

Alternative name
Human acyl-CoA retinol O-fatty-acyltransferase ELISA Kit;Human ARAT ELISA Kit;Human Retinol O-fatty-acyltransferase ELISA Kit;Human diglyceride acyltransferase 2 ELISA Kit;Human GS1999FULL ELISA Kit;Human HMFN1045 ELISA Kit;Human diacylglycerol O-acyltransferase 2 ELISA Kit;Human diacylglycerol O-acyltransferase homolog 2 ELISA Kit;Human diacylglycerol O-acyltransferase-like protein 2 ELISA Kit;
Catalog
E011866
species
Human
GeneDGAT2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman DGAT2 ELISA Kit allows for the in vitro quantitative determination of DGAT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Diacylglycerol O-acyltransferase 2 Protein information
Uniprot ID DGAT2_HUMAN
Uniprot AC Q96PD7; A6ND76; Q5U810; Q68CL3; Q68DJ0; Q8NDB7; Q96BS0; Q9BYE5;
UniGene Hs.129798;
GeneID 84649
KEGG hsa:84649;
Human Diacylglycerol O-acyltransferase 2 Protein SEQUENCE
SEQUENCE 388 AA; 43831 MW; 39EE7783A3F06593 CRC64;
MKTLIAAYSG VLRGERQAEA DRSQRSHGGP ALSREGSGRW GTGSSILSAL
QDLFSVTWLN RSKVEKQLQV ISVLQWVLSF LVLGVACSAI LMYIFCTDCW
LIAVLYFTWL VFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR
MPVLREYLMS GGICPVSRDT IDYLLSKNGS GNAIIIVVGG AAESLSSMPG
KNAVTLRNRK GFVKLALRHG ADLVPIYSFG ENEVYKQVIF EEGSWGRWVQ
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITIPKLEHP
TQQDIDLYHT MYMEALVKLF DKHKTKFGLP ETEVLEVN
UCSC uc001oxa.4; human. [Q96PD7-1];

Category

Human DGAT2 ELISA Kit