Human DIABLO ELISA Kit

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  • Alternative name

    Human direct IAP-binding protein with low pI ELISA Kit;Human second mitochondria-derived activator of caspase ELISA Kit;Human SMAC ELISA Kit;Human DFNA64 ELISA Kit;Human diablo IAP-binding mitochondrial protein ELISA Kit;Human diablo homolog, mitochondrial ELISA Kit;Human diablo-like protein ELISA Kit;

  • Catalog
    E011842
  • species
    Human
  • GeneDIABLO
  • Standard CurveHuman DIABLO ELISA Kit
  • Other Species Human Smac/DIABLO ELISA KitMouse Diablo ELISA KitMouse Smac/DIABLO ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Diablo Homolog (DIABLO). No significant cross-reactivity or interference between Diablo Homolog (DIABLO) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman DIABLO ELISA Kit allows for the in vitro quantitative determination of DIABLO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diablo Homolog (DIABLO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diablo Homolog (DIABLO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diablo Homolog (DIABLO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diablo Homolog (DIABLO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Diablo homolog, mitochondrial Protein information
  • Uniprot ID DBLOH_HUMAN
  • Uniprot AC Q9NR28; B2RDQ0; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6;
  • UniGene Hs.169611;
  • GeneID 56616
  • KEGG hsa:56616;
  • Human Diablo homolog, mitochondrial Protein SEQUENCE
  • SEQUENCE 239 AA; 27131 MW; 70C2AE0DC654D031 CRC64;

    MAALKSWLSR SVTSFFRYRQ CLCVPVVANF KKRCFSELIR PWHKTVTIGF

    GVTLCAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI

    TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY

    LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL

    SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED

  • UCSC uc010tab.4; human. [Q9NR28-1];



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