Human DGP ELISA Kit

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  • Catalog
    E011830
  • species
    Human
  • GeneDGP
  • SpecificityThe microplate is coated with purified deamidated gliadin proteins. The test kit detects antibodies against deamidated gliadin protein epitopes.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe lower detection limits for Anti-DGP IgA were determined at 1.0 U/ml.
  • Intended UseHuman DGP ELISA Kit allows for the in vitro quantitative determination of DGP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyAutoimmune; Immunology; Research Assays; Assay Categories
  • Product Description
    specifical
    Principle of the assay: Deamidated gliadin proteins are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the antigen. Washing of the microwells removes unspecific serum components. Horseradish peroxidase (HRP) conjugated anti-human IgA immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is proportional to the concentration of IgA antibodies present in the original sample.




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