Human AKAP6 ELISA Kit

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  • Alternative name

    Human AKAP-6 ELISA Kit;Human A-kinase anchor protein 100 kDa ELISA Kit;Human AKAP 100 ELISA Kit;Human Protein kinase A-anchoring protein 6 ELISA Kit;Human PRKA6 ELISA Kit;Human mAKAP ELISA Kit;Human AKAP100 ELISA Kit;Human KIAA0311 ELISA Kit;Human ADAP100 ELISA Kit;Human ADAP6 ELISA Kit;Human A-kinase anchoring protein 6 ELISA Kit;Human A-kinase anchor protein 6 ELISA Kit;Human A kinase (PRKA) anchor protein 6 ELISA Kit;Human protein kinase A anchoring protein 6 ELISA Kit;

  • Catalog
    E000118
  • species
    Human
  • GeneAKAP6
  • Other Species Mouse AKAP6 ELISA KitRat Akap6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AKAP6. No significant cross-reactivity or interference between AKAP6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKAP6 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman AKAP6 ELISA Kit allows for the in vitro quantitative determination of AKAP6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This AKAP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AKAP6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||AKAP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKAP6 antibody and an AKAP6-HRP conjugate. The assay sample and buffer are incubated together with AKAP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP6 concentration since AKAP6 from samples and AKAP6-HRP conjugate compete for the anti-AKAP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP6 from the sample, fewer sites are left to bind AKAP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP6 concentration in each sample is interpolated from this standard curve.
  • Human A-kinase anchor protein 6 Protein information
  • Uniprot ID AKAP6_HUMAN
  • Uniprot AC Q13023; A7E242; A7E2D4; O15028;
  • UniGene Hs.509083;
  • GeneID 9472
  • KEGG hsa:9472;
  • Human A-kinase anchor protein 6 Protein SEQUENCE
  • SEQUENCE 2319 AA; 256720 MW; 7607B71AD2140727 CRC64;

    MLTMSVTLSP LRSQDLDPMA TDASPMAINM TPTVEQGEGE EAMKDMDSDQ

    QYEKPPPLHT GADWKIVLHL PEIETWLRMT SERVRDLTYS VQQDSDSKHV

    DVHLVQLKDI CEDISDHVEQ IHALLETEFS LKLLSYSVNV IVDIHAVQLL

    WHQLRVSVLV LRERILQGLQ DANGNYTRQT DILQAFSEET KEGRLDSLTE

    VDDSGQLTIK CSQNYLSLDC GITAFELSDY SPSEDLLSGL GDMTSSQVKT

    KPFDSWSYSE MEKEFPELIR SVGLLTVAAD SISTNGSEAV TEEVSQVSLS

    VDDKGGCEED NASAVEEQPG LTLGVSSSSG EALTNAAQPS SETVQQESSS

    SSHHDAKNQQ PVPCENATPK RTIRDCFNYN EDSPTQPTLP KRGLFLKEET

    FKNDLKGNGG KRQMVDLKPE MSRSTPSLVD PPDRSKLCLV LQSSYPNSPS

    AASQSYECLH KVGNGNLENT VKFHIKEISS SLGRLNDCYK EKSRLKKPHK

    TSEEVPPCRT PKRGTGSGKQ AKNTKSSAVP NGELSYTSKA IEGPQTNSAS

    TSSLEPCNQR SWNAKLQLQS ETSSSPAFTQ SSESSVGSDN IMSPVPLLSK

    HKSKKGQASS PSHVTRNGEV VEAWYGSDEY LALPSHLKQT EVLALKLENL

    TKLLPQKPRG ETIQNIDDWE LSEMNSDSEI YPTYHVKKKH TRLGRVSPSS

    SSDIASSLGE SIESGPLSDI LSDEESSMPL AGMKKYADEK SERASSSEKN

    ESHSATKSAL IQKLMQDIQH QDNYEAIWEK IEGFVNKLDE FIQWLNEAME

    TTENWTPPKA EMDDLKLYLE THLSFKLNVD SHCALKEAVE EEGHQLLELI

    ASHKAGLKDM LRMIASQWKE LQRQIKRQHS WILRALDTIK AEILATDVSV

    EDEEGTGSPK AEVQLCYLEA QRDAVEQMSL KLYSEQYTSS SKRKEEFADM

    SKVHSVGSNG LLDFDSEYQE LWDWLIDMES LVMDSHDLMM SEEQQQHLYK

    RYSVEMSIRH LKKTELLSKV EALKKGGVLL PNDLLEKVDS INEKWELLGK

    TLGEKIQDTM AGHSGSSPRD LLSPESGSLV RQLEVRIKEL KGWLRDTELF

    IFNSCLRQEK EGTMNTEKQL QYFKSLCREI KQRRRGVASI LRLCQHLLDD

    RETCNLNADH QPMQLIIVNL ERRWEAIVMQ AVQWQTRLQK KMGKESETLN

    VIDPGLMDLN GMSEDALEWD EMDISNKLIS LNEESNDLDQ ELQPVIPSLK

    LGETSNEDPG YDEEADNHGG SQYASNITAP SSPHIYQVYS LHNVELYEDN

    HMPFLKNNPK VTGMTQPNVL TKSLSKDSSF SSTKSLPDLL GGSNLVKPCA

    CHGGDMSQNS GSESGIVSEG DTETTTNSEM CLLNAVDGSP SNLETEHLDP

    QMGDAVNVLK QKFTDEGESI KLPNSSQSSI SPVGCVNGKV GDLNSITKHT

    PDCLGEELQG KHDVFTFYDY SYLQGSKLKL PMIMKQSQSE KAHVEDPLLR

    GFYFDKKSCK SKHQTTELQP DVPPHERILA SASHEMDRIS YKSGNIEKTF

    TGMQNAKQLS LLSHSSSIES LSPGGDLFGL GIFKNGSDSL QRSTSLESWL

    TSYKSNEDLF SCHSSGDISV SSGSVGELSK RTLDLLNRLE NIQSPSEQKI

    KRSVSDITLQ SSSQKMSFTG QMSLDIASSI NEDSAASLTE LSSSDELSLC

    SEDIVLHKNK IPESNASFRK RLTRSVADES DVNVSMIVNV SCTSACTDDE

    DDSDLLSSST LTLTEEELCI KDEDDDSSIA TDDEIYEDCT LMSGLDYIKN

    ELQTWIRPKL SLTRDKKRCN VSDEMKGSKD ISSSEMTNPS DTLNIETLLN

    GSVKRVSENN GNGKNSSHTH ELGTKRENKK TIFKVNKDPY VADMENGNIE

    GIPERQKGKP NVTSKVSENL GSHGKEISES EHCKCKALMD SLDDSNTAGK

    EFVSQDVRHL PKKCPNHHHF ENQSTASTPT EKSFSELALE TRFNNRQDSD

    ALKSSDDAPS MAGKSAGCCL ALEQNGTEEN ASISNISCCN CEPDVFHQKD

    AEDCSVHNFV KEIIDMASTA LKSKSQPENE VAAPTSLTQI KEKVLEHSHR

    PIQLRKGDFY SYLSLSSHDS DCGEVTNYIE EKSSTPLPLD TTDSGLDDKE

    DIECFFEACV EGDSDGEEPC FSSAPPNESA VPSEAAMPLQ ATACSSEFSD

    SSLSADDADT VALSSPSSQE RAEVGKEVNG LPQTSSGCAE NLEFTPSKLD

    SEKESSGKPG ESGMPEEHNA ASAKSKVQDL SLKANQPTDK AALHPSPKTL

    TCEENLLNLH EKRHRNMHR

  • UCSC uc001wrq.4; human. [Q13023-1];




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