Human ASIP ELISA Kit

Alternative name
Human ASP ELISA Kit;Human agouti switch protein ELISA Kit;Human AGTI ELISA Kit;Human AGTIL ELISA Kit;Human AGSW ELISA Kit;Human SHEP9 ELISA Kit;Human agouti signaling protein ELISA Kit;Human agouti-signaling protein ELISA Kit;Human agouti signaling protein, nonagouti homolog ELISA Kit;Human nonagouti homolog ELISA Kit;
Catalog
E001179
species
Human
GeneASIP
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ASIP. No significant cross-reactivity or interference between human ASIP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ASIP is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman ASIP ELISA Kit allows for the in vitro quantitative determination of ASIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ASIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ASIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Agouti-signaling protein Protein information
Uniprot ID ASIP_HUMAN
Uniprot AC P42127; Q3SXL2;
UniGene Hs.659995;
GeneID 434
KEGG hsa:434;
Human Agouti-signaling protein Protein SEQUENCE
SEQUENCE 132 AA; 14515 MW; AF82CC3C747F2BE6 CRC64;
MDVTRLLLAT LLVFLCFFTA NSHLPPEEKL RDDRSLRSNS SVNLLDVPSV
SIVALNKKSK QIGRKAAEKK RSSKKEASMK KVVRPRTPLS APCVATRNSC
KPPAPACCDP CASCQCRFFR SACSCRVLSL NC
UCSC uc002xah.2; human.;

Human ASIP ELISA Kit

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