Human DSG3 ELISA Kit

Alternative name
Human 130 kDa pemphigus vulgaris antigen ELISA Kit;Human PVA ELISA Kit;Human cadherin family member 6 ELISA Kit;Human CDHF6 ELISA Kit;Human desmoglein 3 ELISA Kit;Human desmoglein-3 ELISA Kit;
Catalog
E011782
species
Human
GeneDSG3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Desmoglein 3 (DSG3). No significant cross-reactivity or interference between Desmoglein 3 (DSG3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.116ng/mL.
Intended UseHuman DSG3 ELISA Kit allows for the in vitro quantitative determination of DSG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoglein 3 (DSG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoglein 3 (DSG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoglein 3 (DSG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmoglein 3 (DSG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Desmoglein-3 Protein information
Uniprot ID DSG3_HUMAN
Uniprot AC P32926; A8K2V2;
UniGene Hs.1925;
GeneID 1830
KEGG hsa:1830;
Human Desmoglein-3 Protein SEQUENCE
SEQUENCE 999 AA; 107533 MW; 7553CC917E8719BA CRC64;
MMGLFPRTTG ALAIFVVVIL VHGELRIETK GQYDEEEMTM QQAKRRQKRE
WVKFAKPCRE GEDNSKRNPI AKITSDYQAT QKITYRISGV GIDQPPFGIF
VVDKNTGDIN ITAIVDREET PSFLITCRAL NAQGLDVEKP LILTVKILDI
NDNPPVFSQQ IFMGEIEENS ASNSLVMILN ATDADEPNHL NSKIAFKIVS
QEPAGTPMFL LSRNTGEVRT LTNSLDREQA SSYRLVVSGA DKDGEGLSTQ
CECNIKVKDV NDNFPMFRDS QYSARIEENI LSSELLRFQV TDLDEEYTDN
WLAVYFFTSG NEGNWFEIQT DPRTNEGILK VVKALDYEQL QSVKLSIAVK
NKAEFHQSVI SRYRVQSTPV TIQVINVREG IAFRPASKTF TVQKGISSKK
LVDYILGTYQ AIDEDTNKAA SNVKYVMGRN DGGYLMIDSK TAEIKFVKNM
NRDSTFIVNK TITAEVLAID EYTGKTSTGT VYVRVPDFND NCPTAVLEKD
AVCSSSPSVV VSARTLNNRY TGPYTFALED QPVKLPAVWS ITTLNATSAL
LRAQEQIPPG VYHISLVLTD SQNNRCEMPR SLTLEVCQCD NRGICGTSYP
TTSPGTRYGR PHSGRLGPAA IGLLLLGLLL LLLAPLLLLT CDCGAGSTGG
VTGGFIPVPD GSEGTIHQWG IEGAHPEDKE ITNICVPPVT ANGADFMESS
EVCTNTYARG TAVEGTSGME MTTKLGAATE SGGAAGFATG TVSGAASGFG
AATGVGICSS GQSGTMRTRH STGGTNKDYA DGAISMNFLD SYFSQKAFAC
AEEDDGQEAN DCLLIYDNEG ADATGSPVGS VGCCSFIADD LDDSFLDSLG
PKFKKLAEIS LGVDGEGKEV QPPSKDSGYG IESCGHPIEV QQTGFVKCQT
LSGSQGASAL STSGSVQPAV SIPDPLQHGN YLVTETYSAS GSLVQPSTAG
FDPLLTQNVI VTERVICPIS SVPGNLAGPT QLRGSHTMLC TEDPCSRLI
UCSC uc002kws.4; human.;

Category

Human DSG3 ELISA Kit

Human 130 kDa pemphigus vulgaris antigen ELISA Kit;Human PVA ELISA Kit;Human cadherin family member 6 ELISA Kit;Human CDHF6 ELISA Kit;Human desmoglein 3 ELISA Kit;Human desmoglein-3 ELISA Kit;