Human DSG3 ELISA Kit

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  • Alternative name

    Human 130 kDa pemphigus vulgaris antigen ELISA Kit;Human PVA ELISA Kit;Human cadherin family member 6 ELISA Kit;Human CDHF6 ELISA Kit;Human desmoglein 3 ELISA Kit;Human desmoglein-3 ELISA Kit;

  • Catalog
    E011782
  • species
    Human
  • GeneDSG3
  • Standard CurveHuman DSG3 ELISA Kit
  • Other Species Mouse DSG3 ELISA KitCanine DSG3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Desmoglein 3 (DSG3). No significant cross-reactivity or interference between Desmoglein 3 (DSG3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.116ng/mL.
  • Intended UseHuman DSG3 ELISA Kit allows for the in vitro quantitative determination of DSG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoglein 3 (DSG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoglein 3 (DSG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoglein 3 (DSG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmoglein 3 (DSG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Desmoglein-3 Protein information
  • Uniprot ID DSG3_HUMAN
  • Uniprot AC P32926; A8K2V2;
  • UniGene Hs.1925;
  • GeneID 1830
  • KEGG hsa:1830;
  • Human Desmoglein-3 Protein SEQUENCE
  • SEQUENCE 999 AA; 107533 MW; 7553CC917E8719BA CRC64;

    MMGLFPRTTG ALAIFVVVIL VHGELRIETK GQYDEEEMTM QQAKRRQKRE

    WVKFAKPCRE GEDNSKRNPI AKITSDYQAT QKITYRISGV GIDQPPFGIF

    VVDKNTGDIN ITAIVDREET PSFLITCRAL NAQGLDVEKP LILTVKILDI

    NDNPPVFSQQ IFMGEIEENS ASNSLVMILN ATDADEPNHL NSKIAFKIVS

    QEPAGTPMFL LSRNTGEVRT LTNSLDREQA SSYRLVVSGA DKDGEGLSTQ

    CECNIKVKDV NDNFPMFRDS QYSARIEENI LSSELLRFQV TDLDEEYTDN

    WLAVYFFTSG NEGNWFEIQT DPRTNEGILK VVKALDYEQL QSVKLSIAVK

    NKAEFHQSVI SRYRVQSTPV TIQVINVREG IAFRPASKTF TVQKGISSKK

    LVDYILGTYQ AIDEDTNKAA SNVKYVMGRN DGGYLMIDSK TAEIKFVKNM

    NRDSTFIVNK TITAEVLAID EYTGKTSTGT VYVRVPDFND NCPTAVLEKD

    AVCSSSPSVV VSARTLNNRY TGPYTFALED QPVKLPAVWS ITTLNATSAL

    LRAQEQIPPG VYHISLVLTD SQNNRCEMPR SLTLEVCQCD NRGICGTSYP

    TTSPGTRYGR PHSGRLGPAA IGLLLLGLLL LLLAPLLLLT CDCGAGSTGG

    VTGGFIPVPD GSEGTIHQWG IEGAHPEDKE ITNICVPPVT ANGADFMESS

    EVCTNTYARG TAVEGTSGME MTTKLGAATE SGGAAGFATG TVSGAASGFG

    AATGVGICSS GQSGTMRTRH STGGTNKDYA DGAISMNFLD SYFSQKAFAC

    AEEDDGQEAN DCLLIYDNEG ADATGSPVGS VGCCSFIADD LDDSFLDSLG

    PKFKKLAEIS LGVDGEGKEV QPPSKDSGYG IESCGHPIEV QQTGFVKCQT

    LSGSQGASAL STSGSVQPAV SIPDPLQHGN YLVTETYSAS GSLVQPSTAG

    FDPLLTQNVI VTERVICPIS SVPGNLAGPT QLRGSHTMLC TEDPCSRLI

  • UCSC uc002kws.4; human.;



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