Human AGRP ELISA Kit

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  • Alternative name

    Human AGRT ELISA Kit;Human ART ELISA Kit;Human ASIP2 ELISA Kit;Human agouti related neuropeptide ELISA Kit;Human agouti-related protein ELISA Kit;Human agouti related protein homolog ELISA Kit;

  • Catalog
    E001175
  • species
    Human
  • GeneAGRP
  • Standard CurveHuman AGRP ELISA Kit
  • Other Species Mouse Agrp ELISA KitBovine AGRP ELISA KitPorcine AGRP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Agouti Related Protein (AGRP). No significant cross-reactivity or interference between Agouti Related Protein (AGRP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 3.1pg/mL.
  • Intended UseHuman AGRP ELISA Kit allows for the in vitro quantitative determination of AGRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Agouti Related Protein (AGRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Agouti Related Protein (AGRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Agouti Related Protein (AGRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Agouti Related Protein (AGRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Agouti-related protein Protein information
  • Uniprot ID AGRP_HUMAN
  • Uniprot AC O00253; O15459; Q2TBD9;
  • UniGene Hs.104633;
  • GeneID 181
  • KEGG hsa:181;
  • Human Agouti-related protein Protein SEQUENCE
  • SEQUENCE 132 AA; 14440 MW; 1CCBE112C3EB10F5 CRC64;

    MLTAAVLSCA LLLALPATRG AQMGLAPMEG IRRPDQALLP ELPGLGLRAP

    LKKTTAEQAE EDLLQEAQAL AEVLDLQDRE PRSSRRCVRL HESCLGQQVP

    CCDPCATCYC RFFNAFCYCR KLGTAMNPCS RT

  • UCSC uc002etg.1; human.;


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