Human DPT ELISA Kit

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  • Alternative name

    Human tyrosine-rich acidic matrix protein ELISA Kit;Human TRAMP ELISA Kit;Human dermatopontin ELISA Kit;

  • Catalog
    E011744
  • species
    Human
  • GeneDPT
  • Standard CurveHuman DPT ELISA Kit
  • Other Species Mouse Dpt ELISA KitBovine DPT ELISA KitPorcine DPT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dermatopontin (DPT). No significant cross-reactivity or interference between Dermatopontin (DPT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman DPT ELISA Kit allows for the in vitro quantitative determination of DPT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatopontin (DPT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatopontin (DPT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatopontin (DPT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dermatopontin (DPT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dermatopontin Protein information
  • Uniprot ID DERM_HUMAN
  • Uniprot AC Q07507; A8K981; Q8N4R2; Q9UIX8;
  • UniGene Hs.80552;
  • GeneID 1805
  • KEGG hsa:1805;
  • Human Dermatopontin Protein SEQUENCE
  • SEQUENCE 201 AA; 24005 MW; C886D2FB624DDB49 CRC64;

    MDLSLLWVLL PLVTMAWGQY GDYGYPYQQY HDYSDDGWVN LNRQGFSYQC

    PQGQVIVAVR SIFSKKEGSD RQWNYACMPT PQSLGEPTEC WWEEINRAGM

    EWYQTCSNNG LVAGFQSRYF ESVLDREWQF YCCRYSKRCP YSCWLTTEYP

    GHYGEEMDMI SYNYDYYIRG ATTTFSAVER DRQWKFIMCR MTEYDCEFAN

    V

  • UCSC uc001gfp.4; human.;



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