Human DSE ELISA Kit

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  • Alternative name

    Human DS epimerase ELISA Kit;Human chondroitin-glucuronate 5-epimerase ELISA Kit;Human squamous cell carcinoma antigen recognized by T-cells 2 ELISA Kit;Human SART-2 ELISA Kit;Human SART2 ELISA Kit;Human DS-epi1 ELISA Kit;Human DSEP ELISA Kit;Human DSEPI ELISA Kit;Human EDSMC2 ELISA Kit;Human dermatan sulfate epimerase ELISA Kit;Human dermatan-sulfate epimerase ELISA Kit;

  • Catalog
    E011736
  • species
    Human
  • GeneDSE
  • Standard CurveHuman DSE ELISA Kit
  • Other Species Mouse Dse ELISA KitBovine DSE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dermatan Sulfate Epimerase (DSE). No significant cross-reactivity or interference between Dermatan Sulfate Epimerase (DSE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.114ng/mL.
  • Intended UseHuman DSE ELISA Kit allows for the in vitro quantitative determination of DSE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dermatan-sulfate epimerase Protein information
  • Uniprot ID DSE_HUMAN
  • Uniprot AC Q9UL01; Q5R3K6;
  • UniGene Hs.458358; Hs.547713;
  • GeneID 29940
  • KEGG hsa:29940;
  • Human Dermatan-sulfate epimerase Protein SEQUENCE
  • SEQUENCE 958 AA; 109773 MW; A3D05C6194B4D2F9 CRC64;

    MRTHTRGAPS VFFIYLLCFV SAYITDENPE VMIPFTNANY DSHPMLYFSR

    AEVAELQLRA ASSHEHIAAR LTEAVHTMLS SPLEYLPPWD PKDYSARWNE

    IFGNNLGALA MFCVLYPENI EARDMAKDYM ERMAAQPSWL VKDAPWDEVP

    LAHSLVGFAT AYDFLYNYLS KTQQEKFLEV IANASGYMYE TSYRRGWGFQ

    YLHNHQPTNC MALLTGSLVL MNQGYLQEAY LWTKQVLTIM EKSLVLLREV

    TDGSLYEGVA YGSYTTRSLF QYMFLVQRHF NINHFGHPWL KQHFAFMYRT

    ILPGFQRTVA IADSNYNWFY GPESQLVFLD KFVMRNGSGN WLADQIRRNR

    VVEGPGTPSK GQRWCTLHTE FLWYDGSLKS VPPPDFGTPT LHYFEDWGVV

    TYGSALPAEI NRSFLSFKSG KLGGRAIYDI VHRNKYKDWI KGWRNFNAGH

    EHPDQNSFTF APNGVPFITE ALYGPKYTFF NNVLMFSPAV SKSCFSPWVG

    QVTEDCSSKW SKYKHDLAAS CQGRVVAAEE KNGVVFIRGE GVGAYNPQLN

    LKNVQRNLIL LHPQLLLLVD QIHLGEESPL ETAASFFHNV DVPFEETVVD

    GVHGAFIRQR DGLYKMYWMD DTGYSEKATF ASVTYPRGYP YNGTNYVNVT

    MHLRSPITRA AYLFIGPSID VQSFTVHGDS QQLDVFIATS KHAYATYLWT

    GEATGQSAFA QVIADRHKIL FDRNSAIKSS IVPEVKDYAA IVEQNLQHFK

    PVFQLLEKQI LSRVRNTASF RKTAERLLRF SDKRQTEEAI DRIFAISQQQ

    QQQSKSKKNR RAGKRYKFVD AVPDIFAQIE VNEKKIRQKA QILAQKELPI

    DEDEEMKDLL DFADVTYEKH KNGGLIKGRF GQARMVTTTH SRAPSLSASY

    TRLFLILNIA IFFVMLAMQL TYFQRAQSLH GQRCLYAVLL IDSCILLWLY

    SSCSQSQC

  • UCSC uc003pws.5; human.;


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