Human DUT ELISA Kit

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  • Alternative name

    Human dUTPase ELISA Kit;Human dUTP pyrophosphatase ELISA Kit;Human deoxyuridine triphosphatase ELISA Kit;Human deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial ELISA Kit;Human dUTP diphosphatase ELISA Kit;Human dUTP nucleotidohydrolase ELISA Kit;

  • Catalog
    E011713
  • species
    Human
  • GeneDUT
  • Other Species Rat Dut ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman DUT ELISA Kit allows for the in vitro quantitative determination of DUT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human DUT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DUT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DUT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DUT can be calculated.
  • Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial Protein information
  • Uniprot ID DUT_HUMAN
  • Uniprot AC Q96Q81;
  • UniGene Hs.527980;
  • GeneID 1854
  • KEGG hsa:1854;
  • Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial Protein SEQUENCE
  • SEQUENCE 252 AA; 26563 MW; 8E2EBC9ED5B0FAD2 CRC64;

    MTPLCPRPAL CYHFLTSLLR SAMQNARGAR QRAEAAVLSG PGPPLGRAAQ

    HGIPRPLSSA GRLSQGCRGA STVGAAGWKG ELPKAGGSPA PGPETPAISP

    SKRARPAEVG GMQLRFARLS EHATAPTRGS ARAAGYDLYS AYDYTIPPME

    KAVVKTDIQI ALPSGCYGRV APRSGLAAKH FIDVGAGVID EDYRGNVGVV

    LFNFGKEKFE VKKGDRIAQL ICERIFYPEI EEVQALDDTE RGSGGFGSTG

    KN

  • UCSC uc001zws.4; human. [P33316-3];


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