Human DCK ELISA Kit

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  • Alternative name

    Human dCK ELISA Kit;Human deoxycytidine kinase ELISA Kit;Human deoxynucleoside kinase ELISA Kit;

  • Catalog
    E011677
  • species
    Human
  • GeneDCK
  • Standard CurveHuman DCK ELISA Kit
  • Other Species Mouse Dck ELISA KitBovine DCK ELISA KitRat Dck ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Deoxycytidine Kinase (DCK). No significant cross-reactivity or interference between Deoxycytidine Kinase (DCK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman DCK ELISA Kit allows for the in vitro quantitative determination of DCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxycytidine Kinase (DCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxycytidine Kinase (DCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxycytidine Kinase (DCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxycytidine Kinase (DCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Deoxycytidine kinase Protein information
  • Uniprot ID DCK_HUMAN
  • Uniprot AC P27707; B2R8V6; Q5TZY7; Q6FI11;
  • UniGene Hs.709;
  • GeneID 1633
  • KEGG hsa:1633;
  • Human Deoxycytidine kinase Protein SEQUENCE
  • SEQUENCE 260 AA; 30519 MW; 626B9D2D6BED8DBC CRC64;

    MATPPKRSCP SFSASSEGTR IKKISIEGNI AAGKSTFVNI LKQLCEDWEV

    VPEPVARWCN VQSTQDEFEE LTMSQKNGGN VLQMMYEKPE RWSFTFQTYA

    CLSRIRAQLA SLNGKLKDAE KPVLFFERSV YSDRYIFASN LYESECMNET

    EWTIYQDWHD WMNNQFGQSL ELDGIIYLQA TPETCLHRIY LRGRNEEQGI

    PLEYLEKLHY KHESWLLHRT LKTNFDYLQE VPILTLDVNE DFKDKYESLV

    EKVKEFLSTL

  • UCSC uc003hfx.4; human.;


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