Human DSPP ELISA Kit

Alternative name
Human dentin phosphophoryn ELISA Kit;Human DPP ELISA Kit;Human DSP ELISA Kit;Human DFNA39 ELISA Kit;Human DGI1 ELISA Kit;Human DMP3 ELISA Kit;Human dentin sialophosphoprotein ELISA Kit;Human dentin phosphoprotein ELISA Kit;Human dentin phosphoryn ELISA Kit;Human dentin sialoprotein ELISA Kit;
Catalog
E011670
species
Human
GeneDSPP
SpecificityThis assay has high sensitivity and excellent specificity for detection of DSPP. No significant cross-reactivity or interference between DSPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DSPP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman DSPP ELISA Kit allows for the in vitro quantitative determination of DSPP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: DSPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DSPP antibody and an DSPP-HRP conjugate. The assay sample and buffer are incubated together with DSPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSPP concentration since DSPP from samples and DSPP-HRP conjugate compete for the anti-DSPP antibody binding site. Since the number of sites is limited, as more sites are occupied by DSPP from the sample, fewer sites are left to bind DSPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSPP concentration in each sample is interpolated from this standard curve.
Human Dentin sialoprotein Protein information
Uniprot ID DSPP_HUMAN
Uniprot AC Q9NZW4; A8MUI0; O95815;
UniGene Hs.678914;
GeneID 1834
KEGG hsa:1834;
Human Dentin sialoprotein Protein SEQUENCE
SEQUENCE 1301 AA; 131151 MW; E0D86B52F5E53D05 CRC64;
MKIITYFCIW AVAWAIPVPQ SKPLERHVEK SMNLHLLARS NVSVQDELNA
SGTIKESGVL VHEGDRGRQE NTQDGHKGEG NGSKWAEVGG KSFSTYSTLA
NEEGNIEGWN GDTGKAETYG HDGIHGKEEN ITANGIQGQV SIIDNAGATN
RSNTNGNTDK NTQNGDVGDA GHNEDVAVVQ EDGPQVAGSN NSTDNEDEII
ENSCRNEGNT SEITPQINSK RNGTKEAEVT PGTGEDAGLD NSDGSPSGNG
ADEDEDEGSG DDEDEEAGNG KDSSNNSKGQ EGQDHGKEDD HDSSIGQNSD
SKEYYDPEGK EDPHNEVDGD KTSKSEENSA GIPEDNGSQR IEDTQKLNHR
ESKRVENRIT KESETHAVGK SQDKGIEIKG PSSGNRNITK EVGKGNEGKE
DKGQHGMILG KGNVKTQGEV VNIEGPGQKS EPGNKVGHSN TGSDSNSDGY
DSYDFDDKSM QGDDPNSSDE SNGNDDANSE SDNNSSSRGD ASYNSDESKD
NGNGSDSKGA EDDDSDSTSD TNNSDSNGNG NNGNDDNDKS DSGKGKSDSS
DSDSSDSSNS SDSSDSSDSD SSDSNSSSDS DSSDSDSSDS SDSDSSDSSN
SSDSSDSSDS SDSSDSSDSS DSKSDSSKSE SDSSDSDSKS DSSDSNSSDS
SDNSDSSDSS NSSNSSDSSD SSDSSDSSSS SDSSNSSDSS DSSDSSNSSE
SSDSSDSSDS DSSDSSDSSN SNSSDSDSSN SSDSSDSSNS SDSSDSSDSS
NSSDSSDSSD SSNSSDSSDS SDSSDSSDSS NSSDSNDSSN SSDSSDSSNS
SDSSNSSDSS DSSDSSDSDS SNSSDSSNSS DSSDSSNSSD SSDSSDSSDG
SDSDSSNRSD SSNSSDSSDS SDSSNSSDSS DSSDSNESSN SSDSSDSSNS
SDSDSSDSSN SSDSSDSSNS SDSSESSNSS DNSNSSDSSN SSDSSDSSDS
SNSSDSSNSS DSSNSSDSSD SNSSDSSDSS NSSDSSDSSD SSDSSDSSDS
SNSSDSSDSS DSSDSSNSSD SSNSSDSSNS SDSSDSSDSS DSSDSSDSSD
SSDSSNSSDS SDSSDSSDSS DSSDSSDSSD SSESSDSSDS SNSSDSSDSS
DSSDSSDSSD SSDSSDSSDS SNSSDSSDSS DSSDSSDSSN SSDSSDSSES
SDSSDSSDSS DSSDSSDSSD SSDSSDSSNS SDSSDSSDSS DSSDSSDSSD
SSDSSDSSDS SDSSDSSDSS DSSDSSDSSD SNESSDSSDS SDSSDSSNSS
DSSDSSDSSD STSDSNDESD SQSKSGNGNN NGSDSDSDSE GSDSNHSTSD
D
UCSC uc003hqu.3; human.;

Category

Human DSPP ELISA Kit

Human dentin phosphophoryn ELISA Kit;Human DPP ELISA Kit;Human DSP ELISA Kit;Human DFNA39 ELISA Kit;Human DGI1 ELISA Kit;Human DMP3 ELISA Kit;Human dentin sialophosphoprotein ELISA Kit;Human dentin phosphoprotein ELISA Kit;Human dentin phosphoryn ELISA Kit;Human dentin sialoprotein ELISA Kit;