Human DSPP ELISA Kit

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  • Alternative name

    Human dentin phosphophoryn ELISA Kit;Human DPP ELISA Kit;Human DSP ELISA Kit;Human DFNA39 ELISA Kit;Human DGI1 ELISA Kit;Human DMP3 ELISA Kit;Human dentin sialophosphoprotein ELISA Kit;Human dentin phosphoprotein ELISA Kit;Human dentin phosphoryn ELISA Kit;Human dentin sialoprotein ELISA Kit;

  • Catalog
    E011670
  • species
    Human
  • GeneDSPP
  • Standard CurveHuman DSPP ELISA Kit
  • Other Species Mouse Dspp ELISA KitRat Dspp ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DSPP. No significant cross-reactivity or interference between DSPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DSPP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman DSPP ELISA Kit allows for the in vitro quantitative determination of DSPP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: DSPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DSPP antibody and an DSPP-HRP conjugate. The assay sample and buffer are incubated together with DSPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSPP concentration since DSPP from samples and DSPP-HRP conjugate compete for the anti-DSPP antibody binding site. Since the number of sites is limited, as more sites are occupied by DSPP from the sample, fewer sites are left to bind DSPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSPP concentration in each sample is interpolated from this standard curve.
  • Human Dentin sialoprotein Protein information
  • Uniprot ID DSPP_HUMAN
  • Uniprot AC Q9NZW4; A8MUI0; O95815;
  • UniGene Hs.678914;
  • GeneID 1834
  • KEGG hsa:1834;
  • Human Dentin sialoprotein Protein SEQUENCE
  • SEQUENCE 1301 AA; 131151 MW; E0D86B52F5E53D05 CRC64;

    MKIITYFCIW AVAWAIPVPQ SKPLERHVEK SMNLHLLARS NVSVQDELNA

    SGTIKESGVL VHEGDRGRQE NTQDGHKGEG NGSKWAEVGG KSFSTYSTLA

    NEEGNIEGWN GDTGKAETYG HDGIHGKEEN ITANGIQGQV SIIDNAGATN

    RSNTNGNTDK NTQNGDVGDA GHNEDVAVVQ EDGPQVAGSN NSTDNEDEII

    ENSCRNEGNT SEITPQINSK RNGTKEAEVT PGTGEDAGLD NSDGSPSGNG

    ADEDEDEGSG DDEDEEAGNG KDSSNNSKGQ EGQDHGKEDD HDSSIGQNSD

    SKEYYDPEGK EDPHNEVDGD KTSKSEENSA GIPEDNGSQR IEDTQKLNHR

    ESKRVENRIT KESETHAVGK SQDKGIEIKG PSSGNRNITK EVGKGNEGKE

    DKGQHGMILG KGNVKTQGEV VNIEGPGQKS EPGNKVGHSN TGSDSNSDGY

    DSYDFDDKSM QGDDPNSSDE SNGNDDANSE SDNNSSSRGD ASYNSDESKD

    NGNGSDSKGA EDDDSDSTSD TNNSDSNGNG NNGNDDNDKS DSGKGKSDSS

    DSDSSDSSNS SDSSDSSDSD SSDSNSSSDS DSSDSDSSDS SDSDSSDSSN

    SSDSSDSSDS SDSSDSSDSS DSKSDSSKSE SDSSDSDSKS DSSDSNSSDS

    SDNSDSSDSS NSSNSSDSSD SSDSSDSSSS SDSSNSSDSS DSSDSSNSSE

    SSDSSDSSDS DSSDSSDSSN SNSSDSDSSN SSDSSDSSNS SDSSDSSDSS

    NSSDSSDSSD SSNSSDSSDS SDSSDSSDSS NSSDSNDSSN SSDSSDSSNS

    SDSSNSSDSS DSSDSSDSDS SNSSDSSNSS DSSDSSNSSD SSDSSDSSDG

    SDSDSSNRSD SSNSSDSSDS SDSSNSSDSS DSSDSNESSN SSDSSDSSNS

    SDSDSSDSSN SSDSSDSSNS SDSSESSNSS DNSNSSDSSN SSDSSDSSDS

    SNSSDSSNSS DSSNSSDSSD SNSSDSSDSS NSSDSSDSSD SSDSSDSSDS

    SNSSDSSDSS DSSDSSNSSD SSNSSDSSNS SDSSDSSDSS DSSDSSDSSD

    SSDSSNSSDS SDSSDSSDSS DSSDSSDSSD SSESSDSSDS SNSSDSSDSS

    DSSDSSDSSD SSDSSDSSDS SNSSDSSDSS DSSDSSDSSN SSDSSDSSES

    SDSSDSSDSS DSSDSSDSSD SSDSSDSSNS SDSSDSSDSS DSSDSSDSSD

    SSDSSDSSDS SDSSDSSDSS DSSDSSDSSD SNESSDSSDS SDSSDSSNSS

    DSSDSSDSSD STSDSNDESD SQSKSGNGNN NGSDSDSDSE GSDSNHSTSD

    D

  • UCSC uc003hqu.3; human.;



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