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Alternative name
Aggrecan core protein ELISA KIT; Cartilage-specific proteoglycan core protein ELISA KIT; CSPCPACAN ELISA KIT; AGC1 ELISA KIT; CSPG1 ELISA KIT; MSK16 ELISA KIT; CSPCP ELISA KIT; Chondroitin sulfate proteoglycan 1 ELISA KIT
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Catalog
E001163
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species
Human
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GeneAGC
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Standard Curve
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Other Species
Mouse AGC ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Aggrecan (AGC).
No significant cross-reactivity or interference between Aggrecan (AGC) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.65ng/mL.
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Intended UseHuman AGC ELISA Kit allows for the in vitro quantitative determination of AGC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aggrecan (AGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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