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Alternative name
Delta sleep-inducing peptide ELISA KIT; DSIP ELISA KIT; DSIP ELISA KIT
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Catalog
E011622
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species
Human
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GenedSIP
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Standard Curve
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Other Species
Mouse dSIP ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Delta-Sleep Inducing Peptide (dSIP).
No significant cross-reactivity or interference between Delta-Sleep Inducing Peptide (dSIP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 7.15pg/mL.
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Intended UseHuman dSIP ELISA Kit allows for the in vitro quantitative determination of dSIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Delta-Sleep Inducing Peptide (dSIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Delta-Sleep Inducing Peptide (dSIP) and unlabeled Delta-Sleep Inducing Peptide (dSIP) (Standards or samples) with the pre-coated antibody specific to Delta-Sleep Inducing Peptide (dSIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample.
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