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Human AKAP4 ELISA Kit

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  • Alternative name

    Human AKAP-4 ELISA Kit;Human A-kinase anchor protein 82 kDa ELISA Kit;Human AKAP 82 ELISA Kit;Human hAKAP82 ELISA Kit;Human major sperm fibrous sheath protein ELISA Kit;Human HI ELISA Kit;Human Protein kinase A-anchoring protein 4 ELISA Kit;Human PRKA4 ELISA Kit;Human AKAP82 ELISA Kit;Human CT99 ELISA Kit;Human FSC1 ELISA Kit;Human p82 ELISA Kit;Human A-kinase anchoring protein 4 ELISA Kit;Human A-kinase anchor protein 4 ELISA Kit;Human A kinase (PRKA) anchor protein 4 ELISA Kit;Human cancer/testis antigen 99 ELISA Kit;Human protein kinase A anchoring protein 4 ELISA Kit;Human testis-specific gene HI ELISA Kit;
  • Catalog
    E000116
  • species
    Human
  • GeneAKAP4
  • Standard CurveHuman AKAP4 ELISA Kit
  • Other Species Mouse Akap4 ELISA KitRat Akap4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AKAP4. No significant cross-reactivity or interference between AKAP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKAP4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AKAP4 ELISA Kit allows for the in vitro quantitative determination of AKAP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This AKAP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative `determination of Human AKAP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: AKAP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKAP4 antibody and an AKAP4-HRP conjugate. The assay sample and buffer are incubated together with AKAP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP4 concentration since AKAP4 from samples and AKAP4-HRP conjugate compete for the anti-AKAP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP4 from the sample, fewer sites are left to bind AKAP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP4 concentration in each sample is interpolated from this standard curve.
  • Human A-kinase anchor protein 4 Protein information
  • Uniprot ID AKAP4_HUMAN
  • Uniprot AC Q5JQC9; A0AV85; O60904; O95246; Q5JQD1; Q5JQD2; Q5JQD3;
  • UniGene Hs.97633;
  • GeneID 8852
  • KEGG hsa:8852;
  • Human A-kinase anchor protein 4 Protein SEQUENCE

  • SEQUENCE 854 AA; 94477 MW; E440CBE8AB9394F4 CRC64;

    MMAYSDTTMM SDDIDWLRSH RGVCKVDLYN PEGQQDQDRK VICFVDVSTL

    NVEDKDYKDA ASSSSEGNLN LGSLEEKEII VIKDTEKKDQ SKTEGSVCLF

    KQAPSDPVSV LNWLLSDLQK YALGFQHALS PSTSTCKHKV GDTEGEYHRA

    SSENCYSVYA DQVNIDYLMN RPQNLRLEMT AAKNTNNNQS PSAPPAKPPS

    TQRAVISPDG ECSIDDLSFY VNRLSSLVIQ MAHKEIKEKL EGKSKCLHHS

    ICPSPGNKER ISPRTPASKI ASEMAYEAVE LTAAEMRGTG EESREGGQKS

    FLYSELSNKS KSGDKQMSQR ESKEFADSIS KGLMVYANQV ASDMMVSLMK

    TLKVHSSGKP IPASVVLKRV LLRHTKEIVS DLIDSCMKNL HNITGVLMTD

    SDFVSAVKRN LFNQWKQNAT DIMEAMLKRL VSALIGEEKE TKSQSLSYAS

    LKAGSHDPKC RNQSLEFSTM KAEMKERDKG KMKSDPCKSL TSAEKVGEHI

    LKEGLTIWNQ KQGNSCKVAT KACSNKDEKG EKINASTDSL AKDLIVSALK

    LIQYHLTQQT KGKDTCEEDC PGSTMGYMAQ STQYEKCGGG QSAKALSVKQ

    LESHRAPGPS TCQKENQHLD SQKMDMSNIV LMLIQKLLNE NPFKCEDPCE

    GENKCSEPRA SKAASMSNRS DKAEEQCQEH QELDCTSGMK QANGQFIDKL

    VESVMKLCLI MAKYSNDGAA LAELEEQAAS ANKPNFRGTR CIHSGAMPQN

    YQDSLGHEVI VNNQCSTNSL QKQLQAVLQW IAASQFNVPM LYFMGDKDGQ

    LEKLPQVSAK AAEKGYSVGG LLQEVMKFAK ERQPDEAVGK VARKQLLDWL

    LANL

  • UCSC uc004dou.2; human. [Q5JQC9-1];


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