Human DIO1 ELISA Kit

Alternative name
Human 5DI ELISA Kit;Human DIOI ELISA Kit;Human type 1 DI ELISA Kit;Human type-I 5'-deiodinase ELISA Kit;Human ITDI1 ELISA Kit;Human TXDI1 ELISA Kit;Human iodothyronine deiodinase 1 ELISA Kit;Human type I iodothyronine deiodinase ELISA Kit;Human deiodinase, iodothyronine type I ELISA Kit;Human iodothyronine deiodinase type 1 ELISA Kit;Human thyroxine 5'-deiodinase ELISA Kit;Human thyroxine deiodinase type I (selenoprotein) ELISA Kit;
Catalog
E011574
species
Human
GeneDIO1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DIO1. No significant cross-reactivity or interference between DIO1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.054ng/mL.
Intended UseHuman DIO1 ELISA Kit allows for the in vitro quantitative determination of DIO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DIO1 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to DIO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DIO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DIO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DIO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Type I iodothyronine deiodinase Protein information
Uniprot ID IOD1_HUMAN
Uniprot AC Q6Q4C6; Q6Q4C7; Q6Q4C9; Q8WWC6;
UniGene Hs.251415;
GeneID 1733
KEGG hsa:1733;
Human Type I iodothyronine deiodinase Protein SEQUENCE
SEQUENCE 249 AA; 28924 MW; 2D8BBFBA54B5A927 CRC64;
MGLPQPGLWL KRLWVLLEVA VHVVVGKVLL ILFPDRVKRN ILAMGEKTGM
TRNPHFSHDN WIPTFFSTQY FWFVLKVRWQ RLEDTTELGG LAPNCPVVRL
SGQRCNIWEF MQGNRPLVLN FGSCTUPSFM FKFDQFKRLI EDFSSIADFL
VIYIEEAHAS DGWAFKNNMD IRNHQNLQDR LQAAHLLLAR SPQCPVVVDT
MQNQSSQLYA ALPERLYIIQ EGRILYKGKS GPWNYNPEEV RAVLEKLHS
UCSC uc001cwb.4; human. [P49895-1];

Human DIO1 ELISA Kit

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