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Human AGA ELISA Kit

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  • Alternative name

    Human aspartylglucosaminidase ELISA Kit;Human glycosylasparaginase ELISA Kit;Human N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase ELISA Kit;Human AGU ELISA Kit;Human ASRG ELISA Kit;Human GA ELISA Kit;Human N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase ELISA Kit;Human aspartylglucosylamine deaspartylase ELISA Kit;
  • Catalog
    E001151
  • species
    Human
  • GeneAGA
  • Other Species Mouse Aga ELISA KitMonkey AGA ELISA KitPorcine AGA ELISA KitRat Aga ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AGA. No significant cross-reactivity or interference between Human AGA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human AGA and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman AGA ELISA Kit allows for the in vitro quantitative determination of AGA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AGA in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human AGA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AGA. You can calculate the concentration of Human AGA in the samples by comparing the OD of the samples to the standard curve.
  • Human Glycosylasparaginase beta chain Protein information
  • Uniprot ID ASPG_HUMAN
  • Uniprot AC P20933; B2R7H2; D3DP47; Q4W5Q2; Q6FHN6; Q9UCK6; Q9UCK7; Q9UCK8;
  • UniGene Hs.207776;
  • GeneID 175
  • KEGG hsa:175;
  • Human Glycosylasparaginase beta chain Protein SEQUENCE

  • SEQUENCE 346 AA; 37208 MW; 766F1690B5B62FFA CRC64;

    MARKSNLPVL LVPFLLCQAL VRCSSPLPLV VNTWPFKNAT EAAWRALASG

    GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG

    AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA

    SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED

    DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT

    AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF

    FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI

  • UCSC uc003iuu.3; human.;


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