Human DEFA6 ELISA Kit

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  • Alternative name

    Human Defensin, alpha 6 ELISA Kit;Human DEF6 ELISA Kit;Human HD-6 ELISA Kit;Human defensin alpha 6 ELISA Kit;Human defensin-6 ELISA Kit;Human defensin, alpha 6, Paneth cell-specific ELISA Kit;

  • Catalog
    E011495
  • species
    Human
  • GeneDEFA6
  • Standard CurveHuman DEFA6 ELISA Kit
  • Other Species Mouse DEFa6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Alpha 6, Paneth Cell Specific (DEFa6). No significant cross-reactivity or interference between Defensin Alpha 6, Paneth Cell Specific (DEFa6) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.21ng/mL.
  • Intended UseHuman DEFA6 ELISA Kit allows for the in vitro quantitative determination of DEFA6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 6, Paneth Cell Specific (DEFa6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 6, Paneth Cell Specific (DEFa6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 6, Paneth Cell Specific (DEFa6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 6, Paneth Cell Specific (DEFa6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Defensin-6 Protein information
  • Uniprot ID DEF6_HUMAN
  • Uniprot AC Q01524; Q6EZF9;
  • UniGene Hs.711;
  • GeneID 1671
  • KEGG hsa:1671;
  • Human Defensin-6 Protein SEQUENCE
  • SEQUENCE 100 AA; 10975 MW; EDF77E033DDCE2D5 CRC64;

    MRTLTILTAV LLVALQAKAE PLQAEDDPLQ AKAYEADAQE QRGANDQDFA

    VSFAEDASSS LRALGSTRAF TCHCRRSCYS TEYSYGTCTV MGINHRFCCL

  • UCSC uc003wqt.4; human.;



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