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Human DEFA3 ELISA Kit

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  • Alternative name

    Human Defensin, alpha 3 ELISA Kit;Human HNP-3 ELISA Kit;Human HP-3 ELISA Kit;Human HP3 ELISA Kit;Human HNP-2 ELISA Kit;Human HP-2 ELISA Kit;Human HP2 ELISA Kit;Human DEF3 ELISA Kit;Human HNP3 ELISA Kit;Human defensin alpha 3 ELISA Kit;Human neutrophil defensin 3 ELISA Kit;Human defensin 3, neutrophil-specific ELISA Kit;Human defensin, alpha 3, neutrophil-specific ELISA Kit;Human neutrophil peptide 3 ELISA Kit;
  • Catalog
    E011489
  • species
    Human
  • GeneDEFA3
  • Standard CurveHuman DEFA3 ELISA Kit
  • Other Species Mouse Defa3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Alpha 3, Neutrophil Specific (DEFa3). No significant cross-reactivity or interference between Defensin Alpha 3, Neutrophil Specific (DEFa3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.17ng/mL.
  • Intended UseHuman DEFA3 ELISA Kit allows for the in vitro quantitative determination of DEFA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 3, Neutrophil Specific (DEFa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 3, Neutrophil Specific (DEFa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 3, Neutrophil Specific (DEFa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 3, Neutrophil Specific (DEFa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neutrophil defensin 2 Protein information
  • Uniprot ID DEF3_HUMAN
  • Uniprot AC P59666; P11479; Q14125;
  • UniGene Hs.654448;
  • GeneID 1668
  • KEGG hsa:1668;
  • Human Neutrophil defensin 2 Protein SEQUENCE

  • SEQUENCE 94 AA; 10245 MW; 0E0F8E957376C6AF CRC64;

    MRTLAILAAI LLVALQAQAE PLQARADEVA AAPEQIAADI PEVVVSLAWD

    ESLAPKHPGS RKNMDCYCRI PACIAGERRY GTCIYQGRLW AFCC




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