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Human DEFA1B ELISA Kit

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  • Alternative name

    Human Defensin, alpha 1 ELISA Kit;Human HNP-1 ELISA Kit;Human HP-1 ELISA Kit;Human HP1 ELISA Kit;Human HNP-2 ELISA Kit;Human HP-2 ELISA Kit;Human HP2 ELISA Kit;Human defensin alpha 1B ELISA Kit;Human neutrophil defensin 1 ELISA Kit;
  • Catalog
    E011486
  • species
    Human
  • GeneDEFA1B
  • Standard CurveHuman DEFA1B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Alpha 1B (DEFa1B). No significant cross-reactivity or interference between Defensin Alpha 1B (DEFa1B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 14.2pg/mL.
  • Intended UseHuman DEFA1B ELISA Kit allows for the in vitro quantitative determination of DEFA1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 1B (DEFa1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 1B (DEFa1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 1B (DEFa1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 1B (DEFa1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neutrophil defensin 2 Protein information
  • Uniprot ID DEF1_HUMAN
  • Uniprot AC P59665; P11479; Q14125; Q6EZF6;
  • UniGene Hs.380781;
  • GeneID 728358
  • KEGG hsa:728358;
  • Human Neutrophil defensin 2 Protein SEQUENCE

  • SEQUENCE 94 AA; 10201 MW; 0E0F8E95737396FA CRC64;

    MRTLAILAAI LLVALQAQAE PLQARADEVA AAPEQIAADI PEVVVSLAWD

    ESLAPKHPGS RKNMACYCRI PACIAGERRY GTCIYQGRLW AFCC

  • UCSC uc003wqv.2; human.;



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