Human AKR7L ELISA Kit

Alternative name
Human AFB1 aldehyde reductase 3 ELISA Kit;Human AFB1-AR 3 ELISA Kit;Human Aldoketoreductase 7-like ELISA Kit;Human AFAR3 ELISA Kit;Human AKR7A4 ELISA Kit;
Catalog
E001144
species
Human
GeneAKR7L
Standard Curve
Other Species Mouse AKR7L ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKR7L. No significant cross-reactivity or interference between AKR7L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR7L and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AKR7L ELISA Kit allows for the in vitro quantitative determination of AKR7L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This AKR7L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AKR7L. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||AKR7L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR7L antibody and an AKR7L-HRP conjugate. The assay sample and buffer are incubated together with AKR7L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR7L concentration since AKR7L from samples and AKR7L-HRP conjugate compete for the anti-AKR7L antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR7L from the sample, fewer sites are left to bind AKR7L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR7L concentration in each sample is interpolated from this standard curve.
Human Aflatoxin B1 aldehyde reductase member 4 Protein information
Uniprot ID ARK74_HUMAN
Uniprot AC Q8NHP1; Q5U614;
UniGene Hs.662342;
Human Aflatoxin B1 aldehyde reductase member 4 Protein SEQUENCE
SEQUENCE 331 AA; 36964 MW; 6311BEC97EE899F2 CRC64;
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFLYS
DGQSETILGG LGLRMGSSDC RVKIATKANP WIGNSLKPDS VRSQLETSLK
RLQCPUVDLF YLHAPDHSAP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV
AEICTLCKSN GWILPTVYQG MYSATTRQVE TELFPCLRHF GLRFYAYNPL
AGGLLTGKYK YEDKDGKQPV GRFFGTQWAE IYRNHFWKEH HFEGIALVEK
ALQAAYGASA PSMTSAALRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA
AAEEGPLEPA VVDAFNQAWH LFAHECPNYF I
UCSC uc057cwj.1; human. [Q8NHP1-1];

Category

Human AKR7L ELISA Kit

Human AFB1 aldehyde reductase 3 ELISA Kit;Human AFB1-AR 3 ELISA Kit;Human Aldoketoreductase 7-like ELISA Kit;Human AFAR3 ELISA Kit;Human AKR7A4 ELISA Kit;