Human DDX5 ELISA Kit

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  • Alternative name

    Human DEAD box protein 5 ELISA Kit;Human RNA helicase p68 ELISA Kit;Human G17P1 ELISA Kit;Human HELR ELISA Kit;Human HLR1 ELISA Kit;Human HUMP68 ELISA Kit;Human p68 ELISA Kit;Human DEAD-box helicase 5 ELISA Kit;Human probable ATP-dependent RNA helicase DDX5 ELISA Kit;Human ATP-dependent RNA helicase DDX5 ELISA Kit;Human DEAD (Asp-Glu-Ala-Asp) box helicase 5 ELISA Kit;Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 ELISA Kit;Human DEAD box-5 ELISA Kit;Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD) ELISA Kit;

  • Catalog
    E011421
  • species
    Human
  • GeneDDX5
  • Standard CurveHuman DDX5 ELISA Kit
  • Other Species Mouse Ddx5 ELISA KitMonkey DDX5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DEAD Box Polypeptide 5 (DDX5). No significant cross-reactivity or interference between DEAD Box Polypeptide 5 (DDX5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseHuman DDX5 ELISA Kit allows for the in vitro quantitative determination of DDX5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEAD Box Polypeptide 5 (DDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DEAD Box Polypeptide 5 (DDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEAD Box Polypeptide 5 (DDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DEAD Box Polypeptide 5 (DDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Probable ATP-dependent RNA helicase DDX5 Protein information
  • Uniprot ID DDX5_HUMAN
  • Uniprot AC P17844; B4DLW8; B5BU21; D3DU32; E7ETL9; O75681; Q53Y61;
  • UniGene Hs.279806;
  • GeneID 1655
  • KEGG hsa:1655;
  • Human Probable ATP-dependent RNA helicase DDX5 Protein SEQUENCE
  • SEQUENCE 614 AA; 69148 MW; 84DF684FD6871594 CRC64;

    MSGYSSDRDR GRDRGFGAPR FGGSRAGPLS GKKFGNPGEK LVKKKWNLDE

    LPKFEKNFYQ EHPDLARRTA QEVETYRRSK EITVRGHNCP KPVLNFYEAN

    FPANVMDVIA RQNFTEPTAI QAQGWPVALS GLDMVGVAQT GSGKTLSYLL

    PAIVHINHQP FLERGDGPIC LVLAPTRELA QQVQQVAAEY CRACRLKSTC

    IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR RTTYLVLDEA

    DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI

    NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET

    KRRCDELTRK MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV

    ASRGLDVEDV KFVINYDYPN SSEDYIHRIG RTARSTKTGT AYTFFTPNNI

    KQVSDLISVL REANQAINPK LLQLVEDRGS GRSRGRGGMK DDRRDRYSAG

    KRGGFNTFRD RENYDRGYSS LLKRDFGAKT QNGVYSAANY TNGSFGSNFV

    SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VPNMHNGMNQ QAYAYPATAA

    APMIGYPMPT GYSQ

  • UCSC uc002jek.3; human. [P17844-1];


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