Human DDT ELISA Kit

Alternative name
Human D-dopachrome tautomerase ELISA Kit;Human phenylpyruvate tautomerase II ELISA Kit;Human DDCT ELISA Kit;Human D-dopachrome decarboxylase ELISA Kit;
Catalog
E011411
species
Human
GeneDDT
SpecificityThis kit recognizes natural and recombinant Human DDT. No significant cross-reactivity or interference between Human DDT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Human DDT is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseHuman DDT ELISA Kit allows for the in vitro quantitative determination of DDT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DDT. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DDT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DDT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DDT. You can calculate the concentration of DDT in the samples by comparing the OD of the samples to the standard curve.
Human D-dopachrome decarboxylase Protein information
Uniprot ID DOPD_HUMAN
Uniprot AC P30046; B7Z522; O00774; O60787; Q13534;
UniGene Hs.656723; Hs.744172;
GeneID 1652
KEGG hsa:1652;
Human D-dopachrome decarboxylase Protein SEQUENCE
SEQUENCE 118 AA; 12712 MW; 12FEF51908F342B7 CRC64;
MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL
SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF
FPLESWQIGK IGTVMTFL
UCSC uc062cik.1; human. [P30046-1];

Human DDT ELISA Kit

Human D-dopachrome tautomerase ELISA Kit;Human phenylpyruvate tautomerase II ELISA Kit;Human DDCT ELISA Kit;Human D-dopachrome decarboxylase ELISA Kit;

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