Human AFAP1 ELISA Kit

Alternative name
Human 110 kDa actin filament-associated protein ELISA Kit;Human AFAP-110 ELISA Kit;Human AFAP ELISA Kit;Human AFAP110 ELISA Kit;Human actin filament associated protein 1 ELISA Kit;Human actin filament-associated protein 1 ELISA Kit;Human actin filament-associated protein, 110 kDa ELISA Kit;
Catalog
E001137
species
Human
GeneAFAP1
Other Species Mouse Afap1 ELISA Kit Chicken AFAP1 ELISA Kit Rat Afap1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AFAP1. No significant cross-reactivity or interference between Human AFAP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human AFAP1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman AFAP1 ELISA Kit allows for the in vitro quantitative determination of AFAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AFAP1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFAP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human AFAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AFAP1. You can calculate the concentration of Human AFAP1 in the samples by comparing the OD of the samples to the standard curve.
Human Actin filament-associated protein 1 Protein information
Uniprot ID AFAP1_HUMAN
Uniprot AC Q8N556; A8K442; B4DMU2; E9PDT7; Q59EY5; Q9HBY1;
UniGene Hs.529369;
GeneID 60312
KEGG hsa:60312;
Human Actin filament-associated protein 1 Protein SEQUENCE
SEQUENCE 730 AA; 80725 MW; 4E916BB4F82F5547 CRC64;
MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFDVKDHA
QKQETANSLP APPQMPLPEI PQPWLPPDSG PPPLPTSSLP EGYYEEAVPL
SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKTRHQ WPSEEASMDL
VKDAKICAFL LRKKRFGQWT KLLCVIKDTK LLCYKSSKDQ QPQMELPLQG
CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW LKVIKEAYSG
CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE
QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT
CGYLNVLSNS RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG
LDSKHPLTFR LLRNGQEVAV LEASSSEDMG RWIGILLAET GSSTDPEALH
YDYIDVEMSA SVIQTAKQTF CFMNRRVISA NPYLGGTSNG YAHPSGTALH
YDDVPCINGS LKGKKPPVAS NGVTGKGKTL SSQPKKADPA AVVKRTGSNA
AQYKYGKNRV EADAKRLQTK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE
VNAGRKPQAI LEEKLKQLEE ECRQKEAERV SLELELTEVK ESLKKALAGG
VTLGLAIEPK SGTSSPQSPV FRHRTLENSP ISSCDTSDTE GPVPVNSAAV
LKKSQAAPGS SPCRGHVLRK AKEWELKNGT
UCSC uc003gkg.2; human. [Q8N556-1];

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Category

Human AFAP1 ELISA Kit

Human 110 kDa actin filament-associated protein ELISA Kit;Human AFAP-110 ELISA Kit;Human AFAP ELISA Kit;Human AFAP110 ELISA Kit;Human actin filament associated protein 1 ELISA Kit;Human actin filament-associated protein 1 ELISA Kit;Human actin filament-associated protein, 110 kDa ELISA Kit;