Human AFAP1 ELISA Kit

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  • Alternative name

    Human 110 kDa actin filament-associated protein ELISA Kit;Human AFAP-110 ELISA Kit;Human AFAP ELISA Kit;Human AFAP110 ELISA Kit;Human actin filament associated protein 1 ELISA Kit;Human actin filament-associated protein 1 ELISA Kit;Human actin filament-associated protein, 110 kDa ELISA Kit;

  • Catalog
    E001137
  • species
    Human
  • GeneAFAP1
  • Other Species Mouse Afap1 ELISA KitChicken AFAP1 ELISA KitRat Afap1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AFAP1. No significant cross-reactivity or interference between Human AFAP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human AFAP1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman AFAP1 ELISA Kit allows for the in vitro quantitative determination of AFAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AFAP1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFAP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human AFAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AFAP1. You can calculate the concentration of Human AFAP1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Actin filament-associated protein 1 Protein information
  • Uniprot ID AFAP1_HUMAN
  • Uniprot AC Q8N556; A8K442; B4DMU2; E9PDT7; Q59EY5; Q9HBY1;
  • UniGene Hs.529369;
  • GeneID 60312
  • KEGG hsa:60312;
  • Human Actin filament-associated protein 1 Protein SEQUENCE
  • SEQUENCE 730 AA; 80725 MW; 4E916BB4F82F5547 CRC64;

    MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFDVKDHA

    QKQETANSLP APPQMPLPEI PQPWLPPDSG PPPLPTSSLP EGYYEEAVPL

    SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKTRHQ WPSEEASMDL

    VKDAKICAFL LRKKRFGQWT KLLCVIKDTK LLCYKSSKDQ QPQMELPLQG

    CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW LKVIKEAYSG

    CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE

    QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT

    CGYLNVLSNS RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG

    LDSKHPLTFR LLRNGQEVAV LEASSSEDMG RWIGILLAET GSSTDPEALH

    YDYIDVEMSA SVIQTAKQTF CFMNRRVISA NPYLGGTSNG YAHPSGTALH

    YDDVPCINGS LKGKKPPVAS NGVTGKGKTL SSQPKKADPA AVVKRTGSNA

    AQYKYGKNRV EADAKRLQTK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE

    VNAGRKPQAI LEEKLKQLEE ECRQKEAERV SLELELTEVK ESLKKALAGG

    VTLGLAIEPK SGTSSPQSPV FRHRTLENSP ISSCDTSDTE GPVPVNSAAV

    LKKSQAAPGS SPCRGHVLRK AKEWELKNGT

  • UCSC uc003gkg.2; human. [Q8N556-1];


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