Human D2HGDH ELISA Kit

Contact Us
  • Alternative name

    Human D2HGD ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase ELISA Kit;Human D-2-hydroxyglutarate dehydrogenase, mitochondrial ELISA Kit;

  • Catalog
    E011351
  • species
    Human
  • GeneD2HGDH
  • Standard CurveHuman D2HGDH ELISA Kit
  • Other Species Mouse D2hgdh ELISA KitBovine D2HGDH ELISA KitRat D2hgdh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of D2-Hydroxyglutarate Dehydrogenase (D2HGDH). No significant cross-reactivity or interference between D2-Hydroxyglutarate Dehydrogenase (D2HGDH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman D2HGDH ELISA Kit allows for the in vitro quantitative determination of D2HGDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D2-Hydroxyglutarate Dehydrogenase (D2HGDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D2-Hydroxyglutarate Dehydrogenase (D2HGDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D2-Hydroxyglutarate Dehydrogenase (D2HGDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D2-Hydroxyglutarate Dehydrogenase (D2HGDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human D-2-hydroxyglutarate dehydrogenase, mitochondrial Protein information
  • Uniprot ID D2HDH_HUMAN
  • Uniprot AC Q8N465; B4E3L6; E7ENP2; Q6IQ24; Q8N5Q8;
  • UniGene Hs.516813;
  • GeneID 728294
  • KEGG hsa:728294;
  • Human D-2-hydroxyglutarate dehydrogenase, mitochondrial Protein SEQUENCE
  • SEQUENCE 521 AA; 56416 MW; 65D88C4315FA45CE CRC64;

    MLPRRPLAWP AWLLRGAPGA AGSWGRPVGP LARRGCCSAP GTPEVPLTRE

    RYPVRRLPFS TVSKQDLAAF ERIVPGGVVT DPEALQAPNV DWLRTLRGCS

    KVLLRPRTSE EVSHILRHCH ERNLAVNPQG GNTGMVGGSV PVFDEIILST

    ARMNRVLSFH SVSGILVCQA GCVLEELSRY VEERDFIMPL DLGAKGSCHI

    GGNVATNAGG LRFLRYGSLH GTVLGLEVVL ADGTVLDCLT SLRKDNTGYD

    LKQLFIGSEG TLGIITTVSI LCPPKPRAVN VAFLGCPGFA EVLQTFSTCK

    GMLGEILSAF EFMDAVCMQL VGRHLHLASP VQESPFYVLI ETSGSNAGHD

    AEKLGHFLEH ALGSGLVTDG TMATDQRKVK MLWALRERIT EALSRDGYVY

    KYDLSLPVER LYDIVTDLRA RLGPHAKHVV GYGHLGDGNL HLNVTAEAFS

    PSLLAALEPH VYEWTAGQQG SVSAEHGVGF RKRDVLGYSK PPGALQLMQQ

    LKALLDPKGI LNPYKTLPSQ A

  • UCSC uc002wce.3; human. [Q8N465-1];


Related products with Human D2HGDH ELISA Kit

Inquiry About Human D2HGDH ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!