Human AFM ELISA Kit

Alternative name
Human alpha-albumin ELISA Kit;Human alpha-Alb ELISA Kit;Human ALB2 ELISA Kit;Human ALBA ELISA Kit;Human ALF ELISA Kit;Human afamin ELISA Kit;
Catalog
E001133
species
Human
GeneAFM
Other Species Mouse Afm ELISA Kit Rat Afm ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human AFM. No significant cross-reactivity or interference between human AFM and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human AFM is typically less than 1 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.
Intended UseHuman AFM ELISA Kit allows for the in vitro quantitative determination of AFM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Afamin Protein information
Uniprot ID AFAM_HUMAN
Uniprot AC P43652; A8K3E1; Q32MR3; Q4W5C5;
UniGene Hs.168718;
GeneID 173
KEGG hsa:173;
Human Afamin Protein SEQUENCE
SEQUENCE 599 AA; 69069 MW; D594E75E20D308AB CRC64;
MKLLKLTGFI FFLFFLTESL TLPTQPRDIE NFNSTQKFIE DNIEYITIIA
FAQYVQEATF EEMEKLVKDM VEYKDRCMAD KTLPECSKLP NNVLQEKICA
MEGLPQKHNF SHCCSKVDAQ RRLCFFYNKK SDVGFLPPFP TLDPEEKCQA
YESNRESLLN HFLYEVARRN PFVFAPTLLT VAVHFEEVAK SCCEEQNKVN
CLQTRAIPVT QYLKAFSSYQ KHVCGALLKF GTKVVHFIYI AILSQKFPKI
EFKELISLVE DVSSNYDGCC EGDVVQCIRD TSKVMNHICS KQDSISSKIK
ECCEKKIPER GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF
FAKFTFEYSR RHPDLSIPEL LRIVQIYKDL LRNCCNTENP PGCYRYAEDK
FNETTEKSLK MVQQECKHFQ NLGKDGLKYH YLIRLTKIAP QLSTEELVSL
GEKMVTAFTT CCTLSEEFAC VDNLADLVFG ELCGVNENRT INPAVDHCCK
TNFAFRRPCF ESLKADKTYV PPPFSQDLFT FHADMCQSQN EELQRKTDRF
LVNLVKLKHE LTDEELQSLF TNFANVVDKC CKAESPEVCF NEESPKIGN
UCSC uc003hhb.4; human.;

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Category

Human AFM ELISA Kit

Human alpha-albumin ELISA Kit;Human alpha-Alb ELISA Kit;Human ALB2 ELISA Kit;Human ALBA ELISA Kit;Human ALF ELISA Kit;Human afamin ELISA Kit;