Human AFM ELISA Kit

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  • Alternative name

    Human alpha-albumin ELISA Kit;Human alpha-Alb ELISA Kit;Human ALB2 ELISA Kit;Human ALBA ELISA Kit;Human ALF ELISA Kit;Human afamin ELISA Kit;

  • Catalog
    E001133
  • species
    Human
  • GeneAFM
  • Other Species Mouse Afm ELISA KitRat Afm ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human AFM. No significant cross-reactivity or interference between human AFM and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human AFM is typically less than 1 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.
  • Intended UseHuman AFM ELISA Kit allows for the in vitro quantitative determination of AFM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFM bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Afamin Protein information
  • Uniprot ID AFAM_HUMAN
  • Uniprot AC P43652; A8K3E1; Q32MR3; Q4W5C5;
  • UniGene Hs.168718;
  • GeneID 173
  • KEGG hsa:173;
  • Human Afamin Protein SEQUENCE
  • SEQUENCE 599 AA; 69069 MW; D594E75E20D308AB CRC64;

    MKLLKLTGFI FFLFFLTESL TLPTQPRDIE NFNSTQKFIE DNIEYITIIA

    FAQYVQEATF EEMEKLVKDM VEYKDRCMAD KTLPECSKLP NNVLQEKICA

    MEGLPQKHNF SHCCSKVDAQ RRLCFFYNKK SDVGFLPPFP TLDPEEKCQA

    YESNRESLLN HFLYEVARRN PFVFAPTLLT VAVHFEEVAK SCCEEQNKVN

    CLQTRAIPVT QYLKAFSSYQ KHVCGALLKF GTKVVHFIYI AILSQKFPKI

    EFKELISLVE DVSSNYDGCC EGDVVQCIRD TSKVMNHICS KQDSISSKIK

    ECCEKKIPER GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF

    FAKFTFEYSR RHPDLSIPEL LRIVQIYKDL LRNCCNTENP PGCYRYAEDK

    FNETTEKSLK MVQQECKHFQ NLGKDGLKYH YLIRLTKIAP QLSTEELVSL

    GEKMVTAFTT CCTLSEEFAC VDNLADLVFG ELCGVNENRT INPAVDHCCK

    TNFAFRRPCF ESLKADKTYV PPPFSQDLFT FHADMCQSQN EELQRKTDRF

    LVNLVKLKHE LTDEELQSLF TNFANVVDKC CKAESPEVCF NEESPKIGN

  • UCSC uc003hhb.4; human.;


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