Human DAO ELISA Kit

Alternative name
Human DAAO ELISA Kit;Human DAMOX ELISA Kit;Human DAO ELISA Kit;Human OXDA ELISA Kit;Human D-amino acid oxidase ELISA Kit;Human D-amino-acid oxidase ELISA Kit;
Catalog
E011317
species
Human
GeneDAO
Standard Curve
Other Species Mouse Dao ELISA Kit Guinea Pig DAO ELISA Kit Hamster DAO ELISA Kit Monkey DAO ELISA Kit Porcine DAO ELISA Kit Rabbit DAO ELISA Kit Rat Dao ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO). No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.65ng/mL.
Intended UseHuman DAO ELISA Kit allows for the in vitro quantitative determination of DAO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Amino Acid Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Amino Acid Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human D-amino-acid oxidase Protein information
Uniprot ID OXDA_HUMAN
Uniprot AC P14920; B2R7I5; Q16758; Q8N6R2;
UniGene Hs.113227;
GeneID 1610
KEGG hsa:1610;
Human D-amino-acid oxidase Protein SEQUENCE
SEQUENCE 347 AA; 39474 MW; A508E603872F3072 CRC64;
MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG
LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE
AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE
RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG
QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL
NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP
SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL
UCSC uc001tnr.5; human.;

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Category

Human DAO ELISA Kit

Human DAAO ELISA Kit;Human DAMOX ELISA Kit;Human DAO ELISA Kit;Human OXDA ELISA Kit;Human D-amino acid oxidase ELISA Kit;Human D-amino-acid oxidase ELISA Kit;