Human PLA2G4B ELISA Kit

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  • Alternative name

    Human cPLA2-beta ELISA Kit;Human Phospholipase A2 group IVB ELISA Kit;Human HsT16992 ELISA Kit;Human JMJD7-PLA2G4B readthrough ELISA Kit;Human JMJD7-PLA2G4B protein ELISA Kit;Human jumonji domain containing 7-phospholipase A2, group IVB (cytosolic) read-through ELISA Kit;

  • Catalog
    E011273
  • species
    Human
  • GenePLA2G4B
  • Other Species Mouse Pla2g4b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PLA2G4B. No significant cross-reactivity or interference between PLA2G4B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PLA2G4B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PLA2G4B ELISA Kit allows for the in vitro quantitative determination of PLA2G4B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This PLA2G4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLA2G4B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PLA2G4B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PLA2G4B antibody and an PLA2G4B-HRP conjugate. The assay sample and buffer are incubated together with PLA2G4B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLA2G4B concentration since PLA2G4B from samples and PLA2G4B-HRP conjugate compete for the anti-PLA2G4B antibody binding site. Since the number of sites is limited, as more sites are occupied by PLA2G4B from the sample, fewer sites are left to bind PLA2G4B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLA2G4B concentration in each sample is interpolated from this standard curve.
  • Human Cytosolic phospholipase A2 beta Protein information
  • Uniprot ID PA24B_HUMAN
  • Uniprot AC P0C869; B4DRT9; O95712; Q19KD5; Q19KD6; Q59GF9; Q8TB10; Q9UKV7;
  • UniGene Hs.198161;
  • GeneID 8681
  • KEGG hsa:8681;
  • Human Cytosolic phospholipase A2 beta Protein SEQUENCE
  • SEQUENCE 781 AA; 87978 MW; 4C5C6861E7BAB1B8 CRC64;

    MAVAEVSRTC LLTVRVLQAH RLPSKDLVTP SDCYVTLWLP TACSHRLQTR

    TVKNSSSPVW NQSFHFRIHR QLKNVMELKV FDQDLVTGDD PVLSVLFDAG

    TLRAGEFRRE SFSLSPQGEG RLEVEFRLQS LADRGEWLVS NGVLVARELS

    CLHVQLEETG DQKSSEHRVQ LVVPGSCEGP QEASVGTGTF RFHCPACWEQ

    ELSIRLQDAP EEQLKAPLSA LPSGQVVRLV FPTSQEPLMR VELKKEAGLR

    ELAVRLGFGP CAEEQAFLSR RKQVVAAALR QALQLDGDLQ EDEIPVVAIM

    ATGGGIRAMT SLYGQLAGLK ELGLLDCVSY ITGASGSTWA LANLYEDPEW

    SQKDLAGPTE LLKTQVTKNK LGVLAPSQLQ RYRQELAERA RLGYPSCFTN

    LWALINEALL HDEPHDHKLS DQREALSHGQ NPLPIYCALN TKGQSLTTFE

    FGEWCEFSPY EVGFPKYGAF IPSELFGSEF FMGQLMKRLP ESRICFLEGI

    WSNLYAANLQ DSLYWASEPS QFWDRWVRNQ ANLDKEQVPL LKIEEPPSTA

    GRIAEFFTDL LTWRPLAQAT HNFLRGLHFH KDYFQHPHFS TWKATTLDGL

    PNQLTPSEPH LCLLDVGYLI NTSCLPLLQP TRDVDLILSL DYNLHGAFQQ

    LQLLGRFCQE QGIPFPPISP SPEEQLQPRE CHTFSDPTCP GAPAVLHFPL

    VSDSFREYSA PGVRRTPEEA AAGEVNLSSS DSPYHYTKVT YSQEDVDKLL

    HLTHYNVCNN QEQLLEALRQ AVQRRRQRRP H

  • UCSC uc010bco.4; human.;


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