Human DYNC1LI2 ELISA Kit

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  • Alternative name

    Human dynein light intermediate chain 2, cytosolic ELISA Kit;Human LIC-2 ELISA Kit;Human LIC53/55 ELISA Kit;Human DNCLI2 ELISA Kit;Human LIC2 ELISA Kit;Human dynein cytoplasmic 1 light intermediate chain 2 ELISA Kit;Human cytoplasmic dynein 1 light intermediate chain 2 ELISA Kit;Human dynein, cytoplasmic, light intermediate polypeptide 2 ELISA Kit;

  • Catalog
    E011222
  • species
    Human
  • GeneDYNC1LI2
  • Standard CurveHuman DYNC1LI2 ELISA Kit
  • Other Species Mouse Dync1li2 ELISA KitMonkey DYNC1LI2 ELISA KitRat Dync1li2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DYNC1LI2. No significant cross-reactivity or interference between DYNC1LI2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DYNC1LI2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman DYNC1LI2 ELISA Kit allows for the in vitro quantitative determination of DYNC1LI2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: DYNC1LI2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DYNC1LI2 antibody and an DYNC1LI2-HRP conjugate. The assay sample and buffer are incubated together with DYNC1LI2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DYNC1LI2 concentration since DYNC1LI2 from samples and DYNC1LI2-HRP conjugate compete for the anti-DYNC1LI2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DYNC1LI2 from the sample, fewer sites are left to bind DYNC1LI2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DYNC1LI2 concentration in each sample is interpolated from this standard curve.
  • Human Cytoplasmic dynein 1 light intermediate chain 2 Protein information
  • Uniprot ID DC1L2_HUMAN
  • Uniprot AC O43237; A8K6V1; B4DZP4; Q8TAT3;
  • UniGene Hs.369068;
  • GeneID 1783
  • KEGG hsa:1783;
  • Human Cytoplasmic dynein 1 light intermediate chain 2 Protein SEQUENCE
  • SEQUENCE 492 AA; 54099 MW; AF7B4E49E3983DCC CRC64;

    MAPVGVEKKL LLGPNGPAVA AAGDLTSEEE EGQSLWSSIL SEVSTRARSK

    LPSGKNILVF GEDGSGKTTL MTKLQGAEHG KKGRGLEYLY LSVHDEDRDD

    HTRCNVWILD GDLYHKGLLK FAVSAESLPE TLVIFVADMS RPWTVMESLQ

    KWASVLREHI DKMKIPPEKM RELERKFVKD FQDYMEPEEG CQGSPQRRGP

    LTSGSDEENV ALPLGDNVLT HNLGIPVLVV CTKCDAVSVL EKEHDYRDEH

    LDFIQSHLRR FCLQYGAALI YTSVKEEKNL DLLYKYIVHK TYGFHFTTPA

    LVVEKDAVFI PAGWDNEKKI AILHENFTTV KPEDAYEDFI VKPPVRKLVH

    DKELAAEDEQ VFLMKQQSLL AKQPATPTRA SESPARGPSG SPRTQGRGGP

    ASVPSSSPGT SVKKPDPNIK NNAASEGVLA SFFNSLLSKK TGSPGSPGAG

    GVQSTAKKSG QKTVLSNVQE ELDRMTRKPD SMVTNSSTEN EA

  • UCSC uc002eqb.2; human. [O43237-1];


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