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Alternative name
Keratin, type I cytoskeletal 18 ELISA KIT; Cell proliferation-inducing gene 46 protein ELISA KIT; Cytokeratin-18 ELISA KIT; CK-18 ELISA KIT; Keratin-18 ELISA KIT; K18KRT18 ELISA KIT; CYK18 ELISA KIT; PIG46 ELISA KIT; CK-18 ELISA KIT; K18 ELISA KIT
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Catalog
E011162
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species
Human
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GeneCK 18
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SpecificityThis assay has high sensitivity and excellent specificity for detection of CK18. No significant cross-reactivity or interference between CK18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CK18 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman CK 18 ELISA Kit allows for the in vitro quantitative determination of CK 18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCell Biology
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Product Description
specificalIntended Uses: This CK18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CK18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||CK18 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CK18. Standards or samples are then added to the microtiter plate wells and CK18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CK18 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CK18 are added to each well to "sandwich" the CK18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CK18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK18 concentration in each sample is interpolated from this standard curve.
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